How Can We Help?
Skip to content
Researcher looking at a book

Human monoclonal antibody stability in rat cell cultures using surrogate peptide LC-MS/MS

  • Published on June 4, 2019
  • Biologics & Oligonucleotides
  • Test Systems & Methods
  • Tox
  • Scientific Posters

Presented at the 67th ASMS Conference on Mass Spectrometry & Allied Topics in Atlanta, GA


Nadya Galeva, Reed Murbach, Krystal Gilligan, Kevin Westland, Seema Muranjan
SEKISUI XenoTech, LLC, Kansas City, KS, USA


In vitro evaluations of monoclonal antibody (mAb) in drug development can be both useful and challenging. Cytotoxicity and stability of protein therapeutic candidates at relevant concentrations in cell culture are of interest in pharmaceutical research. Bioanalytical strategies for mAb LC-MS based analysis involve using either intact or digested mAb. Our approach for determining the stability of aHuman mAb in cultures of primary rat hepatocytes was to use enzymatic protein digestion followed by LC-MS/MS quantitation of five surrogate peptides…

Request a copy of the poster: