Tritosomes and Lysosomes for Characterization of Biologic Drugs
Presented by Dr. Chris Bohl, Manager of Techical Support, Products
Synopsis
Tritosomes and Lysosomes for Characterization of Biologic Drugs: Investigation of freshly purified rat tritosomes and human hepatic lysosomes as an in vitro tool for characterization of biologic drugs”
The realization of new technologies and the development of targeted biopharmaceutical therapies have accelerated in the last decade with over 30 such compounds currently in clinical trials. With ongoing development and evaluation of strategies, such as antibody drug conjugates, to effectively deliver compounds to targeted cell populations through the endocytic-lysosomal pathway, the development and availability of novel reagents will become paramount. Isolated rat hepatic tritosomes and human hepatic lysosomes are in vitro systems that can be utilized to quickly and conveniently evaluate compound stability and guide development direction of biopharmaceutical candidates. In this study, subcellular isolation techniques combined with immunoblotting, protease arrays, and enzymatic activity assays were carried out to characterize isolated rat tritosomes and human liver lysosomes.
Key Concepts Discussed:
- Isolation techniques and characterization
- Insights into the differences between tritosomes and lysosomes
- In vitro applications
Related Access ADME Scientific Resources:
Scientific Poster: In vitro characterization of Human liver lysosomes isolated from fresh tissue
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