May 2018

Maximize Metabolic Activities by Limiting Hepatocyte Cryoinjury

Author:  Dr. Chris Bohl, Michael Millhollen
Posted:  01 May 2018

Cryopreservation and thawing of primary hepatocytes causes inherent damage to the cells. Traditional pooling methods require cells to undergo two separate cryopreservation and thaw steps, leading to increased cryoinjury and decreased metabolic activity.
 
CryostaX® hepatocytes use a patented process that allows scientists to create primary hepatocyte pools from up to 20 donors with only a single cryopreservation. Individual donor hepatocytes are cryopreserved as CryostaX® pellets, which can then be physically combined and packaged into a single vial without having to thaw and re-cryopreserve a second time. This limits the cryoinjury to each donor, maximizing the metabolic activity of the pool, and removes the extra cost usually associated with custom pooling.
 
Data indicates that minimizing cryoinjury leads to a modest conservation of CYP450 and SULT activities, but has significant advantages when examining UGT activity. When choosing suspension or attaching hepatocytes for your experiments, why choose pools with increased cryoinjury?

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Fig. 1 - The effects of multiple freezes on the activity of phase-1 and phase-2 enzymes in human hepatocytes: Average of four donors frozen and refrozen individually
CryostaX-Hepatocytes-Metabolic-Activity-Fig1.gif

Fig. 2 - The effects of multiple freezes on the activity of phase-1 and phase-2 enzymes in human hepatocytes (pools contain cells from the same donors)
CryostaX-Hepatocytes-Metabolic-Activity-Fig2.gif

Fig. 3 - The effects of multiple freezes on the activity of UDP-glucuronosyl transferases in human hepatocytes (pools contain cells from the same donors)

CryostaX-Hepatocytes-Metabolic-Activity-Fig3.gif

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