Cryoplateable animal hepatocytes, the closest alternative to fresh hepatocytes, are used most often as plated confluent monocultures with a thin overlay of extracellular matrix, which best replicates in vivo cellular function and conditions.
Pooled Plateable Animal Hepatocytes
Available pooled plateable animal hepatocytes
Our Custom Preparations team has extensive experience creating tailored hepatocyte products to meet needs unmet by our standard offerings; see all of our capabilities for cellular and subcellular test systems on our Custom Test Systems page.
Ready-Plate® pre-plated hepatocytes provide a convenient alternative to fresh hepatocytes and solve challenges of thawing and plating in house. We will thaw, plate, overlay with extracellular matrix, and deliver to your door any of our cryopreserved plateable animal hepatocytes in the well-format of your choice.
Utilizing Cryopreserved Plateable Animal Hepatocytes
Our plateable human and animal hepatocytes can be used in monoculture or coculture formats; while monoculture is best suited for observing the activity of enzymes abundant in hepatocytes, cocultures incorporate other cell types and can be used in 2D or 3D systems.
Each lot of attaching hepatocytes is qualified by the ability to maintain a confluent monolayer (>85% confuency) for at least five days (critical for induction and useful for toxicology and some low-turnover compound metabolism studies). Animal lots are characterized using LC-MS/MS analysis for general drug-metabolizing enzyme activity, as listed below. Additionally, a datasheet detailing optimal seeding density and a photomicrograph of culture accompany each lot shipment for user convenience.
- 7-Ethoxycoumarin O-dealkylation
- 7-Hydroxycoumarin glucuronidation
- 7-Hydroxycoumarin sulfonation
- Post-thaw viability and assured minimum yield (AMY)
CryostaX® Pooled Hepatocytes: Greater Convenience, Performance & Versatility
CryostaX® pooled animal hepatocytes present an easy to use, cost-effective, high-value test system for in vitro preclinical studies including metabolic clearance, hepatic uptake transporter assays, and CYP induction screening assays. All of our CryostaX® products are developed using our patented single-freeze cryopreservation method (Patent 9,642,355) which minimizes cryoinjury and promotes high metabolic activity, cell yield, attachment, and viability.
- Patented single-freeze process minimizes cryoinjury
- Custom on-demand pooling based on numerous options at no additional charge
- Large lots
- High-quality data
- Convenient, more efficient thawing process than traditional vial format
- Extensively characterized
- Highly competitive pricing
Thawing & Plating Cryopreserved Hepatocytes
Download our protocol or watch our short demonstration videos below to get the best results when thawing and plating your cryopreserved hepatocytes:
Match your attaching hepatocytes with the right media for optimal results.
OptiThaw and OptiPlate media make it easy to have confidence in your plateable hepatocytes’ success. Specially formulated by our product specialists, our proprietary solutions maximize attachment and viability, giving you reliable results for your in vitro assays. Each of our optimized media products is made to give you the best results from your in vitro test systems. Read our media guide outlining best practices in storage and handling to maximize performance and output of your hepatocyte cultures.