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Hepatocyte Cell Media for Thawing, Incubating, Plating, and Other Applications

To complement the widest selection of hepatocyte products on the market, SEKISUI XenoTech provides optimized hepatocyte media for thawing, incubating, and plating hepatocytes. These cell culture media are uniquely designed to apply to various species and hepatocyte applications and are suggested with any hepatocyte order. For guidance on how to select cell media for your assays, read our media selection guide.

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Available Optimized Cell Media for Primary Hepatocytes

*Scroll down for full descriptions of each product type listed

K8800: Thawing media for rat or mouse CryostaX©

K8100: Thawing media for mouse traditional format cryopreserved hepatocytes

K8500: Thawing media for HepatoSure© 100-donor pooled cryopreserved human hepatocytes

K8000: Thawing media for human or non-rodent animal hepatocytes in any format

K8300: Culture media to help human or animal plateable hepatocytes maintain confluent monolayer

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Available Optimized Cell Media for Immortalized Hepatocytes

Choose optimized media to support high viability and strong cultures for in vitro assays

Optimized Cell Media Specially Formulated for Hepatocytes in Culture or Suspension

  • Improved Attachment Efficiency
  • Extended Expiration Dates
  • Improved Confluency / Morphology
  • Convenient Storage Conditions

OptiThaw:

Contains a nutrient-rich media used in thawing cryopreserved cells. The OptiThaw kit makes it easy to achieve maximum viability and cell yield for each hepatocyte lot. The kit includes cell culture media optimized for thawing cryopreserved hepatocytes plus our OptiCount solution for determining cell yield and viability counts. See our Thawing Demo below for best practices in thawing cryopreserved hepatocytes.

OptiIncubate:

Recommended for both fresh and cryopreserved hepatocyte suspension incubations.

OptiPlate:

Used when plating attachable cryopreserved human, Beagle dog, Cynomolgus monkey, CD1 mouse, Sprague-Dawley rat hepatocytes, etc. It is necessary to re-suspend the cell pellet in OptiPlate hepatocyte media before seeding the cells onto culture dishes as it facilitates hepatocyte attachment efficiency post-thaw. Plateable cells plated in this media will attach to collagen-coated surfaces. See our Plating Demo below for best practices in plating cryopreserved hepatocytes.

OptiCulture:

Recommended for maintaining attachment, viability, and monolayer confluency of plated cryopreserved human, Beagle dog, Cynomolgus monkey, CD1 mouse, Sprague-Dawley rat cultured hepatocytes, as well as fresh plated hepatocytes. This media is recommended for feeding and dosing of cultured hepatocytes.

OptiMatrix:

A convenient hepatocyte overlay matrix that greatly enhances hepatocyte culture functions. The use of OptiMatrix helps to increase confluency, preserve the viability of hepatocyte cultures, as well as maintain the hepatocytes’ normal cuboidal appearance, and well-delineated plasma membrane borders. It also assists in the formation of intact canalicular networks and improved excretory function.

Plating & Thawing

See our demo videos below for proper techniques in thawing and plating cryopreserved hepatocytes:

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Need to know more?

Find a listing of our hepatocyte media kits and how to increase NADPH regeneration for improved results

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