We offer a proprietary Kupffer cell media to assist researchers in culturing cryopreserved Kupffer Cells effectively, maximizing viability and activity.
Specialized Media to Thaw and Culture Kupffer Cells
Kupffer Cell Media
Kupffer cells are resident macrophage, non-parenchymal cells found in the liver and can be used in assays by themselves or with hepatocytes in cocultures to improve the representation of cultures of in vivo conditions. To get the best post-thaw performance out of SEKISUI XenoTech Kupffer cells, we recommend using our specialized Kupffer cell medium, a nutrient-rich formula specially tailored to improve thawing and culturing Kupffer cells.
Utilizing our media to get the most out of your Kupffer cells and cocultures
Pro-inflammatory cytokines released by activated Kupffer cells, such as TNF-α and IL-6, are associated with the up-regulation of acute-phase response proteins and suppression of CYP enzymes1. Our team investigated the potential of Kupffer cell activation in a study where Kupffer cells were isolated from non-transplantable human livers followed by expansion in culture and evaluation of the cytokine response to endotoxin (E. coli lipopolysaccharide, LPS).
- Mallick, Pankajini, et al. “Regulation of Drug-Metabolizing Enzymes in Infectious and Inflammatory Disease: Implications for Biologics–Small Molecule Drug Interactions.” Expert Opinion on Drug Metabolism & Toxicology, vol. 13, no. 6, 2017, pp. 605–616., doi:10.1080/17425255.2017.1292251.