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Immortalized Human Hepatocytes for In Vitro Research and Drug Development

Hepatocytes from our immortalized Fa2N-4 cell line retain near-normal morphology in culture for use in induction screening and lysosomal trapping assays, and allow you to compare results using hepatocytes from the same donor over multiple years and across studies, providing consistent results and the convenience of a predictable supply. Choose from the following product options, cryopreserved or plated on a collagen-coated plate of various well formats:

Our Fa2N-4 Cell Line

The use of fresh human hepatocytes can be problematic due to unpredictable availability, limited supply, and donor-dependent variability in performance. The solution is an immortalized, ready-to-use cell line that is proven to perform well for many in vitro drug development applications.

We offer access to cells from the Fa2N-4 immortalized human hepatocyte cell line, which is useful to evaluate the induction of major cytochrome P450s (CYPs), UDP-glucuronosyltransferases (UGTs), and P-gp, and are a suitable test system for CYP induction screening and lysosomal sequestration/trapping.

Please note that the passaging of these cells is not allowed without a license agreement.

Cell Culture

Use in Lysosomal Trapping assays

View our ADME 101 overview below to learn more about lysomal trapping, an assay to determine affinity of your compound to be sequestered and therefore unavailable for therapeutic activity:

Published Research Using Fa2N-4 Immortalized Hepatocytes

Publication: Lysosomal Sequestration (Trapping) of Lipophilic Amine (Cationic Amphiphilic) Drugs in Immortalized Human Hepatocytes (Fa2N-4 cells)

Poster: The Use of Immortalized Hepatocytes in Induction Studies

Poster: Induction of UDP-Glucuronosyltransferases in Cultures of Fresh and Immortalized Human Hepatocytes

Poster: Cytochrome P450 3A4 and 1A2 Induction in Immortalized and Primary Cultures of Human Hepatocytes

Match your assays to the right test system

Find the right high quality in vitro test systems and reagents for your in-house ADME/DMPK/DDI assays

“Isolation of subcellular fractions: Tissue > Homogenate > S9 > Cytosol > Microsomes

Optimize your plated hepatocyte assays

Our proprietary nutrient-rich media is specifically formulated to give you the best results with our Fa2N-4 immortalized hepatocytes

Multichannel pipette in vitro assay drug compound