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Single Donor Suspension Human Hepatocytes

Single donor suspension human hepatocytes can be used in species comparison, metabolic stability, uptake transporter assays, and other drug metabolism and pharmacokinetics (DMPK)-related assays. We offer assured minimum yield (AMY) and selection criteria options, allowing us to customize your order to meet specific needs of your study.

Similar to our cryoplateable human hepatocytes, individual donors can be selected based on enzyme activity, demographics, and any other characteristics. We offer single donor CroystaX® with an assured minimum yield (AMY) of 5 million cells , or our traditional vial format hepatocytes come with with an AMY of 3, 4, or 6 million cells. Each lot of cryopreserved human hepatocytes is extensively, accurately characterized using LC-MS/MS methods (see table below). Uptake transporter characterization is also available for select lots.

CroystaX® Single Donor Human Hepatocytes

As an alternative to traditional vial format, we offer a convenient format for human and animal cryopreserved primary hepatocytes called CryostaX®. Our patented production technique was developed to minimize the number of freeze-thaw cycles cells have to endure during the pooling process, reducing cryoinjury to improve the quality of your assays. But the resulting format has added benefits; because CryostaX® are cryopreserved in pellet format, AMY per vial can be easily adjusted for customers who need custom cell yields for their assays that require fewer cells. Additionally, the thawing process is simplified (see our demonstration video below) to enable you to go from storage to assay in fewer steps, and our streamlined ordering process gives you all the information you need– including upfront pricing– to choose the perfect lots for your assays.

Hepatocyte Characterization

Enzyme Marker Substrate Reaction
CYP1A2 Phenacetin O-dealkylation
CYP2A6 Coumarin 7-hydroxylation
CYP2B6 Bupropion hydroxylation
CYP2C8 Amodiaquine N-dealkylation
CYP2C9 Diclofenac 4′-hydroxylation
CYP2C19 S-Mephenytoin 4′-hydroxylation
CYP2D6 Dextromethorphan O-demethylation
CYP2E1 Chlorzoxazone 6-hydroxylation
CYP3A4/5 Testosterone 6β-hydroxylation
CYP3A4/5 Midazolam 1′-hydroxylation
 UGT 7-Hydroxycoumarin glucuronidation
SULT 7-Hydroxycoumarin sulfonation
OCT1* 1-Methyl-4-phenylpyridinium iodine
OATP1B1* Estrone-3-sulfate
OATP1B3* Cholecystokinin-8
NTCP* Taurocholic acid

*Uptake transporter characterization available on specified lots

Thawing Cryopreserved Hepatocytes

Download our protocol or watch our short demonstration video below to get the best results when thawing your cryopreserved hepatocytes:

Make sure you get the most out of your hepatocytes

When you purchase from us, we make sure you have all the right tools and information you need to succeed

Coloured SEM of a liver cell (hepatocyte)

Build Your Own Pool

We offer custom pooling for no additional cost! You can select attaching or suspension lots, pool size, donor criteria, enzyme activity characterization, and more

Research scientist in vitro ADME DDI studies