How Can We Help?
Skip to content
test systems

Single Donor SuspensionHuman Hepatocytes

Single donor suspensionHuman hepatocytes can be used in species comparison, metabolic stability, uptake transporter assays, and other drug metabolism and pharmacokinetics (DMPK)-related assays. We offer assured minimum yield (AMY) and selection criteria options, allowing us to customize your order to meet specific needs of your study.

Similar to our cryoplateable human hepatocytes, individual donors can be selected based on enzyme activity, demographics, and any other characteristics. Our standard offerings come with with an assured minimum yield (AMY) of 3, 4, or 6 million cells and each lot is extensively, accurately characterized using LC-MS/MS methods (see table below). Uptake transporter characterization is also available for select lots.

Hepatocyte Characterization

Enzyme Marker Substrate Reaction
CYP1A2 Phenacetin O-dealkylation
CYP2A6 Coumarin 7-hydroxylation
CYP2B6 Bupropion hydroxylation
CYP2C8 Amodiaquine N-dealkylation
CYP2C9 Diclofenac 4′-hydroxylation
CYP2C19 S-Mephenytoin 4′-hydroxylation
CYP2D6 Dextromethorphan O-demethylation
CYP2E1 Chlorzoxazone 6-hydroxylation
CYP3A4/5 Testosterone 6β-hydroxylation
CYP3A4/5 Midazolam 1′-hydroxylation
 UGT 7-Hydroxycoumarin glucuronidation
SULT 7-Hydroxycoumarin sulfonation
OCT1* 1-Methyl-4-phenylpyridinium iodine
OATP1B1* Estrone-3-sulfate
OATP1B3* Cholecystokinin-8
NTCP* Taurocholic acid

*Uptake transporter characterization available on specified lots

Follow our Thawing Demo for best results when using cryopreserved hepatocytes in your in vitro assays

Optimize your assays with the right media

OptiIncubate Hepatocyte Media is specially formulated to maintain higher viability throughout incubation and is recommended for use with suspension hepatocytes for optimal, reproducible results.

Make sure you get the most out of your hepatocytes

When you purchase from us, we make sure you have all the right tools and information you need to succeed

Coloured SEM of a liver cell (hepatocyte)

Build Your Own Pool

We offer custom pooling for no additional cost! You can select attaching or suspension lots, pool size, donor criteria, enzyme activity characterization, and more

Research scientist in vitro ADME DDI studies