We offer pooled hepatic subcellular fractions isolated from liver tissue of CD-1, BALB/c, C57BL/6, and B6C3F1 mouse strains, demonstrating high enzyme activity levels. Options include matching microsomal, S9, and cytosolic fractions from male or female donor pools to minimize variation.
Mouse Liver Microsomes, S9 & Cytosol
Microsomes are supplied in 0.5 mL volume at a concentration of 20 mg/mL in 250 mM sucrose buffer. S9 (1 mL at 20 mg/mL) and cytosol (1 mL at 10 mg/mL) fractions are packaged in a suspension buffer containing 50 mM TRIS-HCI, (pH 7.4 at 4ºC), 150 mM KCI, and 2 mM EDTA.
Utilizing Mouse Liver Subcellular Fractions in Preclinical Research
Pooled mouse liver fractions are used most often in drug metabolism studies for preclinical research defining a drug candidate’s ADME (absorption, distribution, metabolism, and excretion) profile. Our unique preparation methods allow for large donor pools for higher accuracy in average activity levels; large lots for consistency between assays; and availability of matching S9, microsomes, and cytosol for correlation between studies using different test systems.
Microsomes contain the highest concentration of many important drug-metabolizing enzymes including cytochrome P450 (CYP) and UDP-glucuronosyltransferase (UGT) enzymes. Homogenate and S9 fractions contain a mixture of membranes and soluble enzymes that can be further fractionated into the cytosol and microsomes subcellular fractions. Homogenate and S9 express a wide variety of phase I and phase II enzymes and are recommended for many different PK/ADME studies. Microsomal fractions are enriched for phase I enzymes, while Cytosolic fractions contain various soluble drug-metabolizing enzymes and are a recommended test system for evaluating phase II metabolic reactions of a test compound – especially once reactions have been observed in S9 fractions.
Make sure your mouse liver microsomes and S9 fractions are properly activated
For best results from assays using microsomes and S9 fractions, we recommend using NADPH regeneration to support high metabolism levels by ensuring that the NADPH levels are not a limiting factor in your incubations. Our preferred system, RapidStartTM , uses an enzymatic reaction that converts NADP to NADPH, which is then used as a cofactor by CYPs and oxidized back to NADP, and the cycle continues ensuring stable NADPH levels throughout the incubation. RapidStartTM NADPH regeneration is easily activated by simply adding water. Our easy-to-use formula is the most convenient and flexible NADPH regenerating system you’ll find, allowing users to easily tailor the system’s capability by simply varying the amount of water added. RapidStartTM is perfect for long- and short-term incubations and supports the function of recombinant enzymes and subcellular fractions, allowing you to achieve the most accurate and reliable data during your in vitro assays.
Don’t see what you need? Other preparations can be made available!
If you require subcellular test systems not available through our standard product offerings, Custom Subcellular Preparations can be easily ordered to meet the specific needs of your study.
Our designated Custom Products team regularly prepares and isolates cells and/or subcellular fractions from more than 45 different species and has capabilities to meet unique needs that are unavailable through most vendors– non-standard rodent strains, farm animals, insects, precarious tissue isolations such as adrenal gland or jejunum. Our specialists can carry out over 40 characterization assays, providing the end user with a good foundation for their DMPK assays. See our Custom Products page for examples of past custom preparations or get in touch with a specialist to find out how we can meet your needs.