Discovery Plus™ Screening: Cell Permeability (Caco-2 and MKDCKII) Assay
Our Cell Permeability Screening Assay gives you quick, low-cost data for one or more compounds to better understand permeability properties of drug candidates.
The permeability of a compound can affect its absorption and distribution; our Discovery Plus™ Cell Permeability assay makes it possible to measure permeability across either a Caco-2 monolayer to evaluate intestinal absorption or an MDCKII-MDR1 monolayer to evaluate potential blood-brain barrier permeability. Our flexible assay design allows you to measure unidirectional or bidirectional permeability, and inhibitors can be added to evaluate the effect transporters have on the permeability of a drug candidate.
Moving quickly through drug discovery and development saves you time and money; get data from a Discovery Plus™ screening study quickly to make risk-based decisions faster and get you through the pipeline more efficiently.
Our Approach to In Vitro Permeability Screening
- Incubations performed in 24-well transwell plates
- Permeability options include; unidirectional A->B or B->A, bidirectional A->B and B->A, + / – inhibitor
- Options for 1 or 3 incubation time points
- Cultured Caco-2 cells or MDCKII cells
- Incubations performed in triplicate
- Positive control included
- A tabulated summary of results includes:
- Permeability (Papp)
- % recovery
| Cell Line | Positive Control / probe substrate | Positive Control inhibitor |
|---|---|---|
| Caco-2 | digoxin | Valspodar |
| MDCKII-MDR1 | digoxin | Valspodar/Verapamil |
| MDCKII-BCRP | Prazosin | Ko143 |
Permeability data is requested by the FDA for IND submissions
What other in vitro metabolism assays should you be planning next? Get an overview of regulatory expectations for in vitro DDI and metabolism data for IND
What’s Next?
When you’re ready to start contracting IND-enabling preclinical ADME & DDI studies, work with a specialty CRO you can trust to give you high-quality data every time
