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AAALAC Accredited In Vivo Animal ADME Studies

Micro-Autoradiography (mARG) Tissue Distribution Technique

In vivo determination of drug localization in tissue can be uniquely informative to drug developers investigating distribution within the context of the ADME/pharmacokinetic profile of their drug compounds. Micro-autoradiography can demonstrate sites of accumulation of a drug and drug-related material, which could be used as supplemental data for evaluation of parameters such as toxicity, efficacy, drug penetration, or differential metabolite distribution.

Micro-autoradiography histology techniques utilize photographic detection media (nuclear emulsion) to spatially locate drug-derived radioactivity at the cellular level in organ and tissue samples. Therefore, it provides an opportunity for a more detailed examination than Quantitative Whole Body Autoradiography because microscopic radioactivity distribution can be analyzed in target tissues.

Microautoradiography showing Accumulation in the follicles of the thyroid gland the repeated administration

Our Approach to Radiolabeled ADME in Drug Discovery and Development

The Drug Development Solutions Center (DDSC) in Tokai, Japan, has been offering micro-autoradiography (mARG) as a tissue distribution analytical method for more than 30 years, accumulating a wealth of experience in histology, drug deposition, and radioactivity. Like other radiolabeled ADME services, mARG requires a high degree of technical skill and our team at DDSC has extensive experience and training to provide you consistent sections, precise qualitative analysis, and educated interpretation of results. 

 mARG Study Highlights

  • Specialized thaw mount method
  • AAALAC International-accredited facility; 150,000 ft2  campus featuring animal care units
  • Consistent 5 μm slices exposed to nuclear emulsion for radioactive compound visualization 
  • HE stained slides for reference
  • 4-week turnaround time for data/report
Micro-Autoradiography images of mouse Tumor

Micro-autoradiography Methods

Fresh tissue or organ samples are snap-frozen, cryosectioned at 5 μm thickness, and exposed to a nuclear emulsion that has been put on a glass microscope slide for 1–4 weeks. After the exposure, the slides are developed,  stained, and analyzed using a Leica LEITZ LMR light microscope to visually distinguish the localization of the radioactive drug derivative in individual cells.

 Additional Capabilities: Quantitative Comparison

Consecutive 5 μm mARG horizontal skin slices may be considered together to better understand drug penetration in terms of depth. The figure below shows our capabilities to create a histogram from data from multiple skin slices, at 20 μm intervals, to quantify radioactivity at sequential depths.

Drug penetration in skin sections with a histogram to inform quantitative distribution data
Tissue Capabilities Radionuclides Animal Species

Adrenal Gland
Brain
Lung
Liver
Intestine
Kidney
Muscle
Pancreas
Retina
Skin
Spleen
Thyroid Gland
Tumor
Other tissue types may be possible upon request

14C
3H
125I
33P
35S
51Cr
111In
55Fe
59Fe
65Zn
75Se
90Y
153Gd

Rat
Mouse
Rabbit
Dog
Monkey
Miniature Pig
Animal models of human disease
Knock Out Animals
Chimeric Mice with highly humanized liver
Others may be available upon request

Related Service: QWBA

Quantitative Whole Body Autoradiography (QWBA) is another in vivo autoradiography study method in which a rat (or in some cases mouse) is dosed with radiolabeled test article and at successive time points radioactivity is measured in cross-sectioned slides of the whole animal to show distribution over time and achieve critical input data for dosimetry calculations for first in-human (FIH) radiolabeled mass balance studies.

Which Studies & Why:

Not sure which in vivo studies you should plan before your drug goes to first in-human (FIH) clinical trials? Read our quick guide outlining regulatory expectations and basic study outlines for each basic in vivo study we offer

In Vivo Bioanalysis

In Vivo ADME Science

Access ADME™ is your go-to repository for our scientists’ content relevant to radiolabeling and in vivo drug development, including information about regulatory expectations and expertise of our Tokai team

Preclinical data gap analysis