Hepatocyte Media

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Complementing the widest selection of hepatocyte products on the market, Sekisui XenoTech features a selection of hepatocyte media tailored to meet the specific needs of various hepatocyte applications.
 

Try Sekisui XenoTech’s Newly Optimized Hepatocyte Media!

  • Improved Attachment Efficiency
  • Extended Expiration Dates
  • Improved Confluency / Morphology
  • Convenient Storage Conditions
  • Replaced the previous media options on June 1, 2016*

OptiThaw-Logo.gif Hepatocyte Kit (K8000, K8100 & K8500)*
Sekisui XenoTech’s OptiThaw hepatocyte kit contains a nutrient-rich media used in thawing and isolating cryopreserved cells. The OptiThaw kit makes it easy to achieve maximum cell yield and viability for each preparation. The kit includes cell culture media optimized for thawing cryopreserved hepatocytes plus our OptiCount solution for determining cell yield and viability counts. K8500 is included with Sekisui XenoTech's HepatoSure™ 100-donor pooled human hepatocyte product. Watch a video tutorial
*The K8000 OptiThaw Hepatocyte Kit replaced K2000.
*The K8100 OptiThaw Mouse Hepatocyte Kit
replaced K2100.
*The K8500 HepatoSureTM OptiThaw Kit
replaced K2600.

OptiPlate-Logo.gif Hepatocyte Media (K8200)*
Sekisui XenoTech’s OptiPlate media is used when plating attachable and inducible cryopreserved human, Beagle dog, Cynomolgus monkey, CD1 mouse, Sprague-Dawley rat hepatocytes, etc. It is necessary to re-suspend the cell pellet in OptiPlate hepatocyte media before seeding the cells onto culture dishes. Plateable cells resuspended in this media attach to most collagen-coated surfaces. Watch a video tutorial
*K8200 OptiPlate Hepatocyte Media replaced K2200.

OptiCulture-Logo.gif Hepatocyte Media (K8300)*
Sekisui XenoTech’s OptiCulture hepatocyte media is recommended for maintaining attachment of cryopreserved human, Beagle dog, Cynomolgus monkey, CD1 mouse, Sprague-Dawley rat cultured hepatocytes, as well as fresh plated hepatocytes. This media is recommended for feeding and dosing of cultured hepatocytes.
*K8300 OptiCulture Hepatocyte Media replaced K2300.

OptiIncubate-Logo.gif Hepatocyte Media (K8400)*
Sekisui XenoTech’s OptiIncubate hepatocyte media is recommended for both fresh and cryopreserved hepatocyte suspension and plated incubations.
*K8400 OptiIncubate Hepatocyte Media replaced K2500.

OptiMatrix-Logo.gif Hepatocyte Overlay Matrix (K8600K8650)
Human-Cryopreserved-Hepatocytes-OptiMatrix-Comparison-Sml-(1).gifSekisui XenoTech’s OptiMatrix is a convenient and ready to use hepatocyte overlay matrix that greatly enhances hepatocyte cultures. The use of OptiMatrix helps to increase confluency and preserve the viability of hepatocyte cultures as well as maintain the hepatocytes' normal cuboidal appearance, one or two centrally located nuclei, and well-delineated plasma membrane borders. It also assists in the formation of intact canalicular networks and improved excretory function.  

Need help deciding which media you need?


Download our media and reagents flyer for more information. If you have any additional questions, please contact our dedicated product specialists.
  Buy cell and tissue based products
Product Description Amount Use
K8000 OptiThaw Hepatocyte Kit Kit Proprietary cell culture media optimized for thawing cryopreserved hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K8100 OptiThaw Mouse Hepatocyte Kit Kit Proprietary culture media optimized for thawing cryopreserved mouse hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K8200 OptiPlate Hepatocyte Media 40mL Proprietary culture media optimized for resuspending and plating hepatocytes.
K8300 OptiCulture Hepatocyte Media Kit Proprietary culture media optimized for culturing plated hepatocytes. This is a serum-free media. The Pen/Strep supplement is packaged separately for addition when ready to use the media.
K8400 OptIncubate Hepatocyte Media 50mL Culture media optimized for incubation of hepatocytes in suspension and plated incubations.
K8500 Hepatosure OptiThaw Kit Kit Proprietary cell culture media optimized for thawing Hepatosure Pooled cryopreserved hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K8600 Opti Matrix .5mL 0.5mL Hepatocyte overlay matrix to increase confluency, preserve viability, structure and appearance, assist network formation, and improve excretory function.
K8650 Opti Matrix 1.5 mL 1.5mL Hepatocyte overlay matrix to increase confluency, preserve viability, structure and appearance, assist network formation, and improve excretory function.
K4000. MFE Plating Medium F Component A & B 1L Each Proprietary nutrient rich media containing phenol red that is supplemented with serum (component B) prior to use. Media is used for thawing, isolating, and seeding Fa2N-4 cells.
K4001. MFE Plating Medium F Component A & B 100 mL Each Proprietary nutrient rich media containing phenol red that is supplemented with serum (component B) prior to use. Media is used for thawing, isolating, and seeding Fa2N-4 cells.
K4005. MFE Plating Medium F Component A & B 500 mL Each Proprietary nutrient rich media containing phenol red that is supplemented with serum (component B) prior to use. Media is used for thawing, isolating, and seeding Fa2N-4 cells.
K4100.X MFE Essential Support Medium F Kit, 1.0 L Each Proprietary nutrient rich media containing phenol red used for maintaining Fa2N-4 cells in culture. This is a serum free media.
K4105.X MFE Essential Support Medium F Kit, 500 mL Each Proprietary nutrient rich media containing phenol red used for maintaining Fa2N-4 cells in culture. This is a serum free media.
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