Hepatocyte Media

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Complementing the widest selection of hepatocyte products on the market, Sekisui XenoTech features a selection of hepatocyte media tailored to meet the specific needs of various hepatocyte applications.
 

Try Sekisui XenoTech’s Newly Optimized Hepatocyte Media!

  • Improved Attachment Efficiency
  • Extended Expiration Dates
  • Improved Confluency / Morphology
  • Convenient Storage Conditions
  • Replaced the previous media options on June 1, 2016*

OptiThaw-Logo.gif Hepatocyte Kit (K8000, K8100K8500 & K8800)
Sekisui XenoTech’s OptiThaw hepatocyte kit contains a nutrient-rich media used in thawing and isolating cryopreserved cells. The OptiThaw kit makes it easy to achieve maximum cell yield and viability for each preparation. The kit includes cell culture media optimized for thawing cryopreserved hepatocytes plus our OptiCount solution for determining cell yield and viability counts. K8500 is included with Sekisui XenoTech's HepatoSure™ 100-donor pooled human hepatocyte product. Watch a video tutorial

OptiPlate-Logo.gif Hepatocyte Media (K8200)*
Sekisui XenoTech’s OptiPlate media is used when plating attachable and inducible cryopreserved human, Beagle dog, Cynomolgus monkey, CD1 mouse, Sprague-Dawley rat hepatocytes, etc. It is necessary to re-suspend the cell pellet in OptiPlate hepatocyte media before seeding the cells onto culture dishes. Plateable cells resuspended in this media attach to most collagen-coated surfaces. Watch a video tutorial

OptiCulture-Logo.gif Hepatocyte Media (K8300)*
Sekisui XenoTech’s OptiCulture hepatocyte media is recommended for maintaining attachment of cryopreserved human, Beagle dog, Cynomolgus monkey, CD1 mouse, Sprague-Dawley rat cultured hepatocytes, as well as fresh plated hepatocytes. This media is recommended for feeding and dosing of cultured hepatocytes.

OptiIncubate-Logo.gif Hepatocyte Media (K8400)*
Sekisui XenoTech’s OptiIncubate hepatocyte media is recommended for both fresh and cryopreserved hepatocyte suspension and plated incubations.

OptiMatrix-Logo.gif Hepatocyte Overlay Matrix (K8600K8650)
Human-Cryopreserved-Hepatocytes-OptiMatrix-Comparison-Sml-(1).gifSekisui XenoTech’s OptiMatrix is a convenient and ready to use hepatocyte overlay matrix that greatly enhances hepatocyte cultures. The use of OptiMatrix helps to increase confluency and preserve the viability of hepatocyte cultures as well as maintain the hepatocytes' normal cuboidal appearance, one or two centrally located nuclei, and well-delineated plasma membrane borders. It also assists in the formation of intact canalicular networks and improved excretory function.  

Need help deciding which media you need?


Hepatocytes-Media-Guides-sml.jpg

Download our media and reagents flyer for more information. If you have any additional questions, please contact our dedicated product specialists.
  Buy cell and tissue based products
Product Description Amount Use
K8000-WEB OptiThaw Hepatocyte Kit EACH Proprietary cell culture media optimized for thawing cryopreserved hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K8100-WEB OptiThaw Mouse Hepatocyte Kit EACH Proprietary culture media optimized for thawing cryopreserved mouse hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K8200 OptiPlate Hepatocyte Media 40mL Proprietary culture media optimized for resuspending and plating hepatocytes.
K8300 OptiCulture Hepatocyte Media Kit Proprietary culture media optimized for culturing plated hepatocytes. This is a serum-free media. The Pen/Strep supplement is packaged separately for addition when ready to use the media.
K8400 OptiIncubate Hepatocyte Media 50mL Culture media optimized for incubation of hepatocytes in suspension and plated incubations.
K8500-WEB Hepatosure OptiThaw Kit EACH Proprietary cell culture media optimized for thawing Hepatosure Pooled cryopreserved hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K8600 Opti Matrix .5mL 0.5mL Hepatocyte overlay matrix to increase confluency, preserve viability, structure and appearance, assist network formation, and improve excretory function.
K8650 Opti Matrix 1.5 mL 1.5mL Hepatocyte overlay matrix to increase confluency, preserve viability, structure and appearance, assist network formation, and improve excretory function.
K8800-WEB OptiThaw Rodent CryostaX Kit EACH Proprietary culture media optimized for thawing cryopreserved rodent CryostaX hepatocytes, plus Trypan blue solution to obtain accurate cell yield and viability.
K4000-WEB MFE Plating Medium F Component A & B 1L 1 L Proprietary nutrient rich media containing phenol red that is supplemented with serum (component B) prior to use. Media is used for thawing, isolating, and seeding Fa2N-4 cells.
K4001-WEB MFE Plating Medium F Component A & B 100 mL 100mL Proprietary nutrient rich media containing phenol red that is supplemented with serum (component B) prior to use. Media is used for thawing, isolating, and seeding Fa2N-4 cells.
K4005-WEB MFE Plating Medium F Component A & B 500 mL 500mL Proprietary nutrient rich media containing phenol red that is supplemented with serum (component B) prior to use. Media is used for thawing, isolating, and seeding Fa2N-4 cells.
K4100-WEB MFE Essential Support Medium F Kit - 1.0 L 1 L Proprietary nutrient rich media containing phenol red used for maintaining Fa2N-4 cells in culture. This is a serum free media.
K4105-WEB MFE Essential Support Medium F Kit - 500 mL 500ml Proprietary nutrient rich media containing phenol red used for maintaining Fa2N-4 cells in culture. This is a serum free media.
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