ISSX 2011, Poster 209

ISSX 2011, Poster 209

Published:  14 September 2011

Evaluating the potential for lysosomal trapping in immortalized human hepatocytes (Fa2N-4 cells)

Sequestration of high concentrations of lipophilic amines (a.k.a cationic amphiphilic drugs or CADs) in the acidic (pH 4-5) environment of lysosomes contributes to their presystemic clearance (by lysosomal trapping in liver and lung), their large volume of distribution (Vd) and their propensity to cause phospholipidosis (Hanumegowda et al, 2010; Daniel and Wojcikowski, 1999). In a related study with Lysotracker Red, a fluorescent probe that, by virtue of being a lipophilic amine, accumulates in lysosomes, we demonstrated that Fa2N-4 cells (immortalized human hepatocytes) contain functional lysosomes, similar to those observed in non-transformed human hepatocytes (Kazmi et al, 2011).