Comparison of two hepatic uptake assay methods

Comparison of two hepatic uptake assay methods

Published:  12/1/2017 1:34:00 PM

Comparison of two hepatic uptake assay methods, plated and oil layer methods, using human and preclinical species cryopreserved hepatocytes
The prediction of in vivo human hepatic clearance (CL) of drug from in vitro data is important for drug development. Primary human cryopreserved hepatocytes are widely used as an in vitro tool for the prediction of in vivo CL since a variety of hepatic uptake transporters are expressing in them. The oil layer method is a current major hepatic uptake assay method. On the other hand, the plated method has come to be used to evaluate hepatic uptake recently. In this study, we compared the difference between the plated method and the oil layer method using human cryopreserved hepatocytes in order to show the usefulness of the plated method. Also, we examined whether or not the plated method is useful for the evaluation of species difference of hepatic uptake using preclinical species (monkey, dog, and rat) cryopreserved hepatocytes.

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