Ultrasensitive quantitation assay of oligonucleotide therapeutics based on the PALSAR method
Full Title
Ultrasensitive quantitation assay of oligonucleotide therapeutics based on the PALSAR method
Abstract
Recently, development of oligonucleotide therapeutics has accelerated in the pharmaceutical industry, and the needs for ultrasensitive quantitation methods have greatly increased. Conventional approaches, such as hybridization, LC-MS/MS, PCR are used in the analysis. Hybridization is commonly used, yet sensitivity is insufficient. Sensitivity of LC-MS/MS is improved by instrumental advancement, but still not enough. PCR and qRT-PCR are also known as sensitive detection methods; however, the high variability of measured values is a problem. To solve these problems, we aimed to develop an ultrasensitive hybridization assay method without nucleic acid amplification procedure.