Human mAb stability and cytotoxicity in rat hepatocytes with a surrogate peptide approach

Human mAb stability and cytotoxicity in rat hepatocytes with a surrogate peptide approach

Published:  7/30/2019 12:00:00 PM

Evaluating stability and cytotoxicity of human monoclonal antibody in cultured rat hepatocytes with a surrogate peptide approach

Presented at the 12th International ISSX Meeting in Portland, OR
Nadya Galeva, Reed Murbach, Krystal Gilligan, Kevin Westland, Seema Muranjan and Joanna E. Barbara
Sekisui XenoTech, LLC, Kansas City, KS, USA

In vitro cell based assays are performed throughout the drug development process. Probing cytotoxicity and stability of a drug candidate at relevant concentrations in cell culture are of interest in pharmaceutical research. Building upon the research presented at the 2019 ASMS Conference, we evaluated the stability of a human monoclonal antibody (mAb) in cultures of primary rat hepatocytes using enzymatic protein digestion and LC-MS quantitation of surrogate peptides to develop and optimize a method and demonstrate applicability of the surrogate peptide approach to in vitro assays for biopharmaceuticals...
 

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