Application of Hepatocytes for In Vitro Uptake Assays

Application of Hepatocytes for In Vitro Uptake Assays

Aired:  2/6/2013 10:00:00 AM

This webinar provides a basic review of concepts applicable to a structure-based approach for the evaluation of hepatic uptake with in vitro hepatocyte experiments.

Key concepts discussed in this webinar will include:

  • Selection of appropriate in vitro study type and design based on predictions from a drug candidate's phys-chem properties
  • Description of the basic in vitro methodologies using hepatocytes, both suspension and plated, to evaluate drug uptake, namely whole-system versus medium loss, oil-spin method and uptake into plated cells
  • Discussion of the practical challenges associated with these experiments
  • Basic review on the estimation of active and passive uptake components from these in vitro hepatocyte experiments
  • Impact of phys-chem properties, active and passive uptake and metabolism on the estimation of free intracellular drug concentrations
  • Brief review of the prediction of in vivo hepatic clearance from in vitro uptake experiments and potential scaling techniques

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