Enzyme Induction



Overview

Sekisui XenoTech's Discovery Plus™ Induction is more than just a screening service. Our 96-well plate format allows up to 10 NCEs at 3 concentrations and positive controls in triplicate wells. Discovery Plus™ Induction is a non-GLP service that provides customers with CYP3A4 mRNA fold change data in less than 10 days. This service is best utilized for compounds that are in the early discovery / development phase. For compounds that will be submitted for regulatory agency approval, please see our definitive CYP Induction service.

Discovery Plus™ Induction uses Sekisui XenoTech's specialized screening design to evaluate CYP3A4 fold change in mRNA. This service yields data presented in a tabular summary of fold change, compared to the vehicle for CYP3A4 (other CYPs are available). Pre-characterized cryopreserved primary hepatocytes are treated in a 96-well plate format for ~24 hours with vehicle, NCEs at 3 concentrations and positive control(s) in triplicate wells. RNA is analyzed utilizing qRT-PCR technology by the cDNA ddCt method (samples normalized to endogenous house keeping gene). The fold induction is then calculated as test compound over the vehicle control. Our Discovery Plus™ design offers the most value in the industry, providing you important data quickly, at a very attractive price point.

Discovery Plus™ Induction Overview
  • Measures mRNA fold induction in CYP3A4 (other CYPs may be substituted)
  • Utilizes qRT-PCR techniques for mRNA measurement
  • Design allows up to 10 NCEs at 3 concentrations and positive controls, performed in triplicate
  • Data presented in tabular summary of fold induction
  • Offered as a non-GLP service
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Protein Binding
Metabolic Stability
CYP Inhibition
Drug Transporter Inhibition
Cell-Based Permeability
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