Cytotoxicity

Sekisui XenoTech's cytotoxicity studies can assess the risk that a test article may cause toxicity in cells, particularly human, non-human or immortalized human hepatocytes (hepatotoxicity). Critical factors that we consider when performing such studies include:
  • Confluent, morphologically normal cells that have been allowed to establish cell-to-cell contact
  • Examining potential toxicity by parent compound(s)
  • Experimental conditions that allow for metabolism-dependent toxicity to be observed
  • Analyzing preparations from multiple humans due to high inter-individual variability (when using human hepatocytes)

It is important to measure endpoints that are well-characterized and understood, carefully choose concentrations of test articles, and treat cells with different concentrations in order to generate concentration-response profiles for early-stage compounds. Sekisui XenoTech performs the LDH (lactate dehydrogenase)-release assay, Alamar Blue assay and qRT-PCR analysis of mRNA to assess toxicity in vitro. This information is then used to generate concentration-response profiles with pharmacologically-relevant concentrations of test articles. In addition, any morphological changes of the cultures are documented with photomicrographs for reference.
  Find the Services You Need  
How Can We Help You?
(please note: pressing Enter will submit the form)
Or Call 1-877-588-7530
(+1.913.438.7450 If Outside North America)


<< Enzyme Induction
In vitro CYP Induction (Definitive)
In vitro UGT Induction (Definitive)
In vitro Induction Express (Screening)
Ex vivo CYP and UGT Enzyme Induction
Contact Us

Contact Us

Questions or inquiries? Use our contact form and we’ll reply as soon as possible.* indicates required field

Request A Quote
Request A Quote

Request A Quote

 Security code