Cell-Based Permeability



Overview

The permeability of a compound can affect its absorption and distribution. Sekisui XenoTech's Discovery Plus™ Cell-Based Permeability assay makes it possible to measure permeability across a monolayer Caco-2 to evaluate intestinal absorption or across MDCKII-MDR1 cells to evaluate blood brain barrier permeability. Our flexible assay design allows you to measure unidirectional or bidirectional permeability and inhibitors can be added to evaluate the effect transporters have on the permeability.

Discovery Plus™ Cell-Based Permeability Overview
  • Incubations performed in 24-well transwell plates
  • Permeability options include; unidirectional A->B or B->A, bidirectional A->B and B->A, + / - inhibitor
  • Options for 1 or 3 incubation time points
  • Caco-2 cells cultured for 19-25 days, MDCKII cells cultured for 3-6 days
  • Incubations performed in triplicate
  • Positive control included
 
Cell Line Positive Control /
probe substrate
Positive Control inhibitor
Caco-2 digoxin Valspodar
MDCKII-MDR1 digoxin Valspodar / Verapamil
MDCKII-BCRP Prazosin Ko143

Discovery Plus™ Cell-Based Permeability Results
A tabulated summary of the following will be provided:
  • pmol the crossed the membrane
  • permeability (Papp)
  • % recovery
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