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Test Systems & Methods

As test systems become introduced and subsequently vetted by CROs and the scientific community for value and utility in quality, predictive assays, we compile relevant studies and research relating to those which are commonly used or suggested by regulatory authorities for in-house testing. To view our available test systems and reagents, visit our DMPK Test Systems page.

 

Webinars

Dual-Hybridization PALSAR (Probe Alternation Link Self-Assembly Reaction) Technology Introduction

Presenter: Yuya Ono, MSc, MBA. Sekisui Medical, Co. Ltd. The quantitative bioanalysis of oligonucleotide therapeutics is mainly conducted with LC-MS/MS and hybridization-based ligand binding assays (LBA)....
Blog

FAQ: Using Plateable Hepatocytes in In Vitro Assays

Plateable hepatocytes are often used for induction studies and to research metabolism, biochemistry, virology, host/pathogen interactions, and cell biology. Here,...

Scientific Posters

Establishment of PALSAR method for quantification of human cells in mouse kidney

Full Title Establishment of PALSAR method for quantification of human cells in mouse kidney Authors Tetsuo Sekino, Akane Omori and AkiraIdeno Analytical Technology Center for...
Scientific Posters

Highly selective and sensitive hybridization-based assay for quantification of ASOs drug using signal amplification method

Full Title Highly selective and sensitive hybridization-based assay for quantification of ASOs drug using signal amplification method Authors Yuya Ono1, Tetsuo Sekino1, Takuro Akiya1, Funa...
Scientific Posters

A Novel ADA Screening Assay for Immunogenicity Testing of Oligonucleotide Drug Using PALSAR Technology

Full Title A Novel ADA Screening Assay for Immunogenicity Testing of Oligonucleotide Drug Using PALSAR® Technology Authors Masako Osawa, Funa Ogawa, Koichi Shibusawa and Akira...
Scientific Posters

Investigation of the PPB method for oligonucleotide drugs by using the PALSAR

Full Title Investigation of the PPB method for oligonucleotide drugs by using the PALSAR® Authors Rieko Sakai1, Takashi Yamamoto1, Masako Osawa2, Funa Ogawa2, Shun Kumagaya3,...
Blog

Which Hepatocytes Should I Use for What Studies?

Primary hepatocytes are considered the gold standard for ADME/DMPK studies because they are the most representative in vitro test system. However, not all hepatocyte formats…

Webinars

Reactive Metabolite Detection Studies: Cysteine Trapping

Presenter: Miki Fujishima, Manager in Business Development at the Drug Development Solutions Center in Tokai, Japan. Abstract: Drug-induced liver injury (DILI) is caused by various...
Blog

Why Do Most Polled Researchers Run Red Blood Cell Partitioning Studies with Plasma Protein Binding?

Many compounds bind to or diffuse into red blood cells (RBCs), which can significantly impact clearance and cause inaccuracies in PK calculations...

Blog

Notes from the 2021 HRA Expert Panel Discussion “Are Standard Hepatocyte Models/Test Systems Still Good Enough?”

MicroPhysiological Systems (MPS) is a general term used to describe miniaturized, in vitro test systems that couple microfluidics with advanced...

Scientific Posters

Establishment of the PPB method for antisense oligonucleotides (ASOs) by using the PALSAR method

Full Title Establishment of the PPB method for antisense oligonucleotides (ASOs) by using the PALSAR® method Authors Rieko Sakai1, Takashi Yamamoto1, Masako Osawa2, Funa Ogawa2,...
Webinars

Addressing Unique Challenges with Custom In Vitro Test Systems

Presenter: Zell Woodworth, Division Director of Products at XenoTech Abstract: Sometimes, standard products just don’t fit the objective of a unique assay. In this webinar, Products...
Webinars

The Role of In Vitro Assays in Selecting the Right Species for Your Small Molecule Program

Presenters: Andrew G. Taylor, Ph.D., Manager of Technical Support for Services at XenoTech and Scott Boley, Ph.D., DABT, Senior Vice President of Research at Sinclair Research...
Blog

Why Switch to Hepatocyte Pellets?

You may have heard about the patented CryostaX® format of hepatocyte pellets, but do you know why XenoTech and countless other researchers have made the…

Webinars

Red Blood Cell Partitioning Studies to Improve Accuracy in Pharmacokinetics (PK) Calculations

Presenter: Steven McGreal, Ph.D, Study Director at XenoTech Abstract: Pharmacokinetic (PK) parameters of a new drug candidate are typically determined by measuring the drug’s concentration...
Blog

Meet the Scientist: Stephanie Helmstetter

As part of our Meet the Scientist series, we introduce you to one of our most notable product experts, Stephanie Helmstetter.

Webinars

In Vitro Cholestatic DILI & Mitochondrial Toxicity Studies to Assess Hepatotoxicity

Drug-Induced Liver Injury (DILI) incidents account for more than 10% of all cases of acute liver failure, posing a major clinical and regulatory challenge. While the cause of DILI is multifactorial and difficult to predict, there are known mechanisms...
Publications

An FGF15/19-TFEB regulatory loop controls hepatic cholesterol and bile acid homeostasis

Bile acid synthesis plays a key role in regulating whole body cholesterol homeostasis. Transcriptional factor EB (TFEB) is a nutrient and stress-sensing transcriptional factor that...
Webinars

ADME 101: Biobank Fatty Liver Disease Tissues and Their Exploration in Microarrays

Presenter: Maciej Czerwinski, Ph.D., XenoTech Director of Consulting This ADME 101 discusses biobank tissue and its research applications. The Research Biobank was established to preserve,...
Blog

Drug-Drug Interaction (DDI) Prediction Models Following In Vitro Studies in Preclinical Development

In preclinical development, a drug will be evaluated for potential to cause a drug-drug interaction (DDI) using in vitro experiments and then calculations that...

Blog

Toxicokinetic (TK) Analysis for Preclinical Drug Development

The main goal of preclinical toxicokinetic (TK) studies is to establish a correlation between a candidate compound’s concentration or dose...

Webinars

ADME 101: Which Hepatocyte Test System Should I Use?

Presented by Chris Bohl, Ph.D., Global Technical Support Manager This ADME 101 video provides an overview of different types of hepatocytes, their unique benefits, and...
Scientific Posters

Toxicity evaluation by high content analysis of 3D cultured primary human hepatocytes

Full Title Toxicity evaluation by high content analysis of 3D cultured primary human hepatocytes Authors Toshimasa Ito, Kazuhiro Fujimoto, Ryo Fujino, Masato Kobayashi, Miki Fujishima,...
Webinars

ADME 101: Guide to Subcellular Fractions and When to Use Which Test System

Presenter:  Chris Bohl, Ph.D., Global Technical Support Manager This ADME 101 video provides an overview of different types of subcellular fractions, such as microsomes, S9,...
Blog

New TMPRSS2-Expressing Cell Line for COVID-19 Research

We are offering a brand new cell line to assist researchers in the fight against COVID-19.The cell line (JCRB1819), developed...

Webinars

ADME 101 FAQ: Tips & Tricks for Working with Plateable Hepatocytes

Presenter: Halee McElhaney, XenoTech Team Lead, Quality Control In the latest installment of ADME 101, one of our hepatocyte experts answers frequently asked questions about plateable...
Publications

Effects of monocyte chemoattractant protein-1, macrophage inflammatory protein-1α, and interferon-α2a on P450 enzymes in human hepatocytes in vitro

Maciej Czerwiński, Krystal Gilligan, Kevin Westland, Brian W. Ogilvie Some immunomodulatory agents stimulate the release of cytokines capable of suppressing P450 enzymes and potentially affecting pharmacokinetics of coadministered medications. Cytokines...
Blog

How to Choose the Right Test Systems for Your DMPK Studies

Test systems for DMPK in vitro studies are part of the very foundation of our company. Our labs were borne of...

Scientific Posters

CYP1A2 enzyme activity and protein abundance in normal and diseased pediatric livers

Full Title CYP1A2 enzyme activity and protein abundance in normal and diseased pediatric livers Authors M. Czerwinski, B. Ewy, A. Kats, M. T. Pritchard, S....
Scientific Posters

CYP1A2 enzyme localization in normal and diseased pediatric livers

Presented at the 55th Congress of European Societies of Toxicology (EUROTOX 2019) in Helsinki, Finland Full Poster Title “CYP1A2 Enzyme Activity and Protein Abundance In Normal...
Blog

Cypex Adds Recombinant UGTs

Recombinant enzymes represent a useful test system for studies requiring extra high levels of catalytic activity. Due to high customer demand, Cypex has...

Scientific Posters

Human mAb stability and cytotoxicity in rat hepatocytes with a surrogate peptide approach

Evaluating stability and cytotoxicity of human monoclonal antibody in cultured rat hepatocytes with a surrogate peptide approach Presented at the 12th International ISSX Meeting in Portland...
Blog

Cell Handling & Media Selection for Best Results in Hepatocyte Assays

Getting reliable, reproducible results from studies using hepatocytes as a model system for drug metabolism is in part dependent on how well...

Scientific Posters

Human monoclonal antibody stability in rat cell cultures using surrogate peptide LC-MS/MS

Evaluating stability of human monoclonal antibody in rat cell cultures using a surrogate peptide LC-MS/MS approach Presented at the 67th ASMS Conference on Mass Spectrometry &...
Blog

Quality Control is What Makes the Comprehensive Collection of Cell Lines from JCRB Among the Highest Regarded and Most Widely Distributed in the World

About JCRB cell bank In addition to focus on preclinical ADME in vitro testing services and complementary products, XenoTech offers to our clients...

Blog

Minding Your Binding: Plasma Protein Binding Potential Study Now Available at Our US Labs

While liver and intestine are important in ADME, behavior in blood is also crucial. When an orally administered drug or...

Scientific Posters

Patterns of hyaluronan deposition in normal and diseased pediatric livers

Full Title Patterns of hyaluronan deposition in normal and diseased pediatric livers Authors Maciej Czerwiński1, Alexander Kats2, Jordan Surgnier3, Sarah E. Tague4, and Michele T....
Blog

Disease-State Test Systems

XenoTech has been working with and supplying reagents for the pre-clinical ADME field for well over two decades. Years of...

Blog

Important Considerations for the Conduct of In Vitro Drug Transporter Assays

Not only can drug transporters affect the absorption and excretion of drugs, they can be involved in pharmacokinetic-based drug-drug interactions...

Scientific Posters

Alcohol consumption uniquely increases hepatic hyaluronan content in patients with liver steatosis

Full Title Alcohol consumption uniquely increases hepatic hyaluronan content in patients with liver steatosis Authors Dr. Maciej Czerwinski1, Jordan Surgnier2, Dr. Sarah E. Tague3 and...
Scientific Posters

Ultrasensitive quantitation assay of oligonucleotide therapeutics based on the PALSAR method

Full Title Ultrasensitive quantitation assay of oligonucleotide therapeutics based on the PALSAR method Abstract Recently, development of oligonucleotide therapeutics has accelerated in the pharmaceutical industry,...
Scientific Posters

Inhibitory effect of typical CYP inhibitors under long term incubation in human liver microsomes

Full Title Inhibitory effect of typical CYP inhibitors under long term incubation in human liver microsomes Authors Sho Nishinoaki, Ryo Fujino, Kenta Hashizume, Tsutomu Negama...
Blog

To GLP or not to GLP?

That is the question. . . Knowing the answer may save you time and money Good Laboratory Practices (GLP) are...

Webinars

CryostaX Pellets: Improving hepatocyte pooling, activity and lab efficiency

Presenter:  Chris Bohl, Ph.D., Global Technical Support Manager Synopsis: CryostaX® hepatocytes are created using a patented process that produces unique single-donor cell pellets. This format allows for distinct benefits...
Blog

Cypex Expands Portfolio of Recombinant Enzymes

As a distributor for Cypex in the US and Canada, XenoTech is proud to offer this portfolio of products based on patented...

Blog

New Rodent, Monkey CryostaX Hepatocytes Featured in Industry News

Following XenoTech’s announcement XenoTech Adds Monkey, Rodent Hepatocytes to Patented CryostaX Product Line, Anticipates the End of Traditionally Cryopreserved Hepatocytes, various...

Scientific Posters

Microsomal cytochrome P450 (CYP) enzyme activities in nonalcoholic steatohepatitis (NASH) livers

Full Title Pitfalls in the Design of Metabolism-Dependent CYP Inhibition (MDI) Experiments With a Dilution Step: Inhibitor Depletion by Metabolism and/or Microsomal Binding Leads to...
Blog

No-Cost, On-Demand Primary Human Hepatocyte Pooling Using CryostaX Pellets

CryostaX® hepatocytes are created using a patented process that produces unique single-donor cell pellets. This format allows scientists to easily pool primary...

Blog

NADPH RapidStart Regeneration System for Extended Metabolism

Originally published in tebu-bio’s Being Bioreactive. To purchase XenoTech’s products in the EU, please visit tebu-bio’s website. NADPH is a critical cofactor...

Webinars

Patient-Derived Tumor Organoids for Personalized Medicine & Targeted Drug Discovery

Presenter: Motoki Takagi, Ph.D., Professor, Medical-industrial Translational Research Center, Fukushima Medical University Synopsis: Patient-derived tumor organoids (PDOs) are cell aggregates produced by in vitro cell...
Blog

Maximize Metabolic Activities by Limiting Hepatocyte Cryoinjury

Cryopreservation and thawing of primary hepatocytes causes inherent damage to the cells. Traditional pooling methods require cells to undergo two separate cryopreservation...

Blog

Assess Catabolic Stability of Biologics & ADCs with Lysosomes – Characterized Test Systems

Originally published in tebu-bio’s Being Bioreactive. To purchase XenoTech’s products in the EU, please visit tebu-bio’s website.  Following up on my series...

Blog

No More Water Baths! Simplify Hepatocyte Thaws and Eliminate Contamination

XenoTech has patented technology that eliminates the need for the water baths that are traditionally required to thaw cryopreserved hepatocytes. By removing water...

Blog

Choosing a Relevant Small Animal Model for Pharmacokinetic or Toxicity Studies

Choosing the most suitable small animal model is crucial for pharmacokinetic and/or toxicology studies in order to get usable, relevant data. Due to potential...

Blog

Recombinant CYP Bactosomes: Versatile Formats for Many ADME Applications

Originally published in tebu-bio’s Being Bioreactive. To purchase recombinant CYPs in the EU, please visit tebu-bio’s website. For North American orders, please follow...

Blog

Insects and Cattle and Sheep, Oh My!

Over the past two decades, we’ve received a lot of interesting requests for custom tissue preparations here at XenoTech. “We’ve...

Publications

In Vitro Products & Reagents Technology Guide

The Product Technology Guide provides a complete list of XenoTech’s products as well as their uses and applications. This guide also has a comprehensive appendix...
Blog

What To Do with Microsome Stable Low Turnover Compounds

Compounds that exhibit high metabolic stability in hepatocytes and subcellular fractions (S9, microsomes and cytosol) can be a challenge for ADME scientists. These in...

Scientific Posters

Comparison of two hepatic uptake assay methods

Full Title “Comparison of two hepatic uptake assay methods, plated and oil layer methods, using  human and preclinical species cryopreserved hepatocytes” Authors Akito Takahashi, Ryota...
Blog

HepatoSure Hepatocytes: The Largest Donor Pool For Your DMPK Applications

Pooled human hepatocytes can complement a selection of hepatocytes from various species for metabolite profiling, species comparison and evaluation of potential human-specific metabolites. HepatoSure® is the...

Blog

Cold Storage Solution Linked to Lower AO, XO Activity

At the time of organ recovery, human livers that have been donated for research are flushed with an ice-cold perfusion...

Blog

Considerations When Studying Esterase Activities in the Intestine

The gastrointestinal wall is a significant site of first-pass metabolism for oral drugs, which is commonly associated with CYP450 and...

Blog

New range of Pig P450s Bactosomes

If you are looking for enzymatic in vitro tools for drug metabolism, reaction phenotyping or metabolite generation using a pig model, you may find these newly...

Blog

New Liver Disease Resource and Other Research Biobank News

XenoTech is committed to furthering the knowledge surrounding hepatic diseases, which affects one out of four people worldwide, such as...

Blog

Big Hepatocyte News!

As you may have heard, XenoTech was issued U.S. Patent No. 9,642,355 for the “CRYOPRESERVATION OF CELLS AND SUBCELLULAR FRACTIONS” for its CryostaX® hepatocytes. The...

Scientific Posters

Hepatocytes as the preferred test system to evaluate oligonucleotide-CYP Interactions in vitro

Full Title Hepatocytes as the preferred test system to evaluate oligonucleotide-CYP Interactions in vitro Abstract XenoTech previously examined the potential of oligonucleotides to inhibit cytochrome...
Blog

XenoTech Adds New Dermal Subcellular Fraction Test Systems

XenoTech is adding human and minipig to the company’s list of species with dermal subcellular fractions available as standard test systems for the development...

Scientific Posters

Research collection of variants of normal and fatty liver disease in human livers

Full Title Research collection of variants of normal and fatty liver disease in human livers Abstract XenoTech has developed a Research Biobank that is a...
Scientific Posters

Evaluation of the CYP metabolic activities in the presence of albumin in human liver microsomes

Full Title Evaluation of the CYP metabolic activities in the presence of albumin in human liver microsomes Abstract Cytochrome P450 (CYP) isoforms are responsible for...
Scientific Posters

Evaluation method for mitochondrial toxicity using Crabtree effect

Full Title Evaluation method for mitochondrial toxicity using Crabtree effect Abstract Mitochondria plays a crucial role in living organisms, one of which being the synthesis...
Blog

XenoTech Standardizes Lysosomal Test Systems for Biopharmaceutical Development

Before releasing these test systems, there was not a high quality, specific, relevant, easy and ready to use, in vitro test system to...

Webinars

Adverse drug interaction risks in genetically-defined subpopulations

Presenter:  Maciej Czerwinski, Ph.D., Director of XenoTech Products R&D Synopsis: An increased appreciation of the effects of genetic variability on the pharmacokinetic and pharmacodynamic properties of xenobiotics creates a...
Webinars

Tritosomes and Lysosomes for Characterization of Biologic Drugs

Presented by Dr. Chris Bohl, Manager of Techical Support, Products Synopsis Tritosomes and Lysosomes for Characterization of Biologic Drugs: Investigation of freshly purified rat tritosomes...
Scientific Posters

Automated High Content Drug Transport Screening with Transwell Plates for Drug Discovery

Full Title Automated High Content Drug Transport Screening with Transwell Plates for Drug Discovery Abstract The purpose of this study was to develop a method...
Scientific Posters

In vitro characterization of human liver lysosomes isolated from fresh tissue

Full Title In vitro characterization of human liver lysosomes isolated from fresh tissue Abstract Evaluation of lysosomal catabolism is an integral part of the development...
Scientific Posters

Efficient use of chimeric mice with humanized livers for drug development support

Full Title Efficient use of chimeric mice with humanized livers for drug development support Abstract Chimeric mice with humanized livers can be used to obtain...
Scientific Posters

Comparison of Ki and IC50 Values for Prototypical Inhibitors of ABC Transporters

Full Title Comparison of Ki and IC50 Values for Prototypical Inhibitors of the Human ABC Transporters P-gp and BCRP in Membrane Vesicles Abstract P-gp (ABCB1/MDR1) and...
Scientific Posters

Comparison of Ki and IC50 Values for Prototypical Inhibitors of ABC Transporters P-gp and BCRP

Full Title Comparison of Ki and IC50 Values for Prototypical Inhibitors of ABC Transporters P-gp and BCRP Abstract Ki values were determined for prototypical inhibitors...
Scientific Posters

Automated Plasma Protein Binding Using Rapid Equilibrium Dialysis

Full Title Automated Plasma Protein Binding Using Rapid Equilibrium Dialysis Abstract A pharmacokinetic/pharmacodynamic principle important in drug development is that it is the free (unbound)...
Scientific Posters

Rapid mRNA Induction Screen

Full Title A Rapid High Content Cytochrome P450 (CYP) mRNA Induction Screen for Early Drug Development Abstract Cytochrome P450 (CYP) enzymes play an important role...
Scientific Posters

Effects of Various Media on the In Vitro Metabolism of Cytochrome P450 Substrates

Full Title The Effect of Buffer Ionic Strength or Various Media on the In Vitro Metabolism of Cytochrome P450 Substrates in Pooled Human Liver Microsomes...
Scientific Posters

Assessment Under Initial Rate Conditions of the Selectivity and Time Course of Cytochrome P450 Inactivation in Pooled Human Liver Microsomes and Hepatocytes

Full Title Assessment Under Initial Rate Conditions of the Selectivity and Time Course of Cytochrome P450 Inactivation in Pooled Human Liver Microsomes and Hepatocytes: Optimization...
Scientific Posters

Comparison of Ki and IC50 Values for Prototypical Inhibitors of the Major Drug Uptake Transporters

Full Title Comparison of Ki and IC50 Values for Prototypical Inhibitors of the Major Drug Uptake Transporters Abstract We previously reported on the similarities and...
Scientific Posters

In Vitro System-Dependent Inhibition of CYPs, UGTs, and Drug Transporters by Oligonucleotides

Full Title In Vitro System-Dependent Inhibition of CYPs, UGTs, and Drug Transporters by Oligonucleotides Abstract We previously examined the cytochrome P450 (CYP) inhibition potential of...
Scientific Posters

The Impact of Incomplete Dose-Response Curves on EC50 and Emax Determinations in Enzyme Induction

Full Title The Impact of Incomplete Dose-Response Curves on EC50 and Emax Determinations in Enzyme Induction Assessment Abstract In evaluating the enzyme induction potential of...
Scientific Posters

The Use of Pooled Plated Cryopreserved Human Hepatocytes for the Determination of Metabolic Clearance, CYP Induction, and Uptake Transporter Studies

Full Title The Use of Pooled Plated Cryopreserved Human Hepatocytes for the Determination of Metabolic Clearance, CYP Induction, and Uptake Transporter Studies Abstract During early...
Scientific Posters

High content automated metabolic stability and CYP inhibition cocktail screening assays for early drug development

Full Title High content automated metabolic stability and CYP inhibition cocktail screening assays for early drug development Abstract The assessment of metabolic stability and cytochrome...
Scientific Posters

Human liver S9 fractions stored at -70°C maintain high phase I and phase II enzymatic activities over multiple freeze/thaw cycles and for at least 10 years 

Full Title Human liver S9 fractions stored at -70°C maintain high phase I and phase II enzymatic activities over multiple freeze/thaw cycles and for at...
Scientific Posters

Endotoxin up-regulates the proinflammatory cytokines TNF-α and IL-6 in freshly-isolated human Kupffer cells

Full Title Endotoxin up-regulates the proinflammatory cytokines TNF-α and IL-6 in freshly-isolated human Kupffer cells Abstract Kupffer cells, macrophages endogenous to the liver, can modulate...
Scientific Posters

Comparing Ki and IC50 Values

Full Title Comparison of Ki and IC50 values for prototypical and clinically-relevant probe substrates of the hepatic transporters OATP1B1 and OATP1B3 Abstract Organic anion transporting polypeptides 1B1...
Webinars

Aldehyde Oxidase: Why In Vitro Absolute Activity Doesn’t Matter

Presented by: Brian Ogilvie and Faraz Kazmi Biotransformation by aldehyde oxidase (AO) is an important clearance mechanism for many drugs and drug candidates, with increasing...
Publications

Exploring the Utility of High-Resolution MS with Post-Acquisition Data Mining for Simultaneous Exogenous and Endogenous Metabolite Profiling

Exploring the Utility of High-Resolution MS with Post-Acquisition Data Mining for Simultaneous Exogenous and Endogenous Metabolite Profiling Presented by: Barbara JE, Buckley DB, Horrigan MJ...
Publications

Lysosomal Sequestration (Trapping) of Lipophilic Amine (Cationic Amphiphilic) Drugs in Immortalized Human Hepatocytes (Fa2N-4 cells)

Kazmi F, Hensley T, Pope C, Funk RS, Loewen GJ, Buckley DB, Parkinson A Lipophilic (logP > 1) and amphiphilic drugs (also known as cationic...
Webinars

Application of Hepatocytes for In Vitro Uptake Assays

Presented by: David Buckley, Ph.D. – CSO This webinar provides a basic review of concepts applicable to a structure-based approach for the evaluation of hepatic...
Publications

High-Resolution Mass Spectrometry Elucidates Metabonate (False Metabolite) Formation from Alkylamine Drugs During In Vitro Metabolite Profiling

Presented by: Barbara JE, Kazmi F, Muranjan S, Toren PC, Parkinson A In vitro metabolite profiling and characterization experiments are widely employed in early drug...
Scientific Posters

A robust method to identify compounds that undergo intracellular lysosomal sequestration

Full Title A robust method to identify compounds that undergo intracellular lysosomal sequestration Abstract Lysosomes are acidic organelles (pH 4-5) that play a key role...
Scientific Posters

Evaluation of dilution, dialysis and ultracentrifugation methods to assess the reversibility of metabolism-dependent inhibitors (MDIs) of cytochrome P450 (CYP) enzymes

Full Title Evaluation of dilution, dialysis and ultracentrifugation methods to assess the reversibility of metabolism-dependent inhibitors (MDIs) of cytochrome P450 (CYP) enzymes Abstract Metabolism-dependent inhibition...
Scientific Posters

Temporal changes in CYP3A4 mRNA and activity following treatment of cultured human hepatocytes with interleukin-6 (IL-6): Implications for study design and endpoint selection

Full Title Temporal changes in CYP3A4 mRNA and activity following treatment of cultured human hepatocytes with interleukin-6 (IL-6): Implications for study design and endpoint selection...
Scientific Posters

Evaluating the potential for lysosomal trapping in immortalized human hepatocytes (Fa2N-4 cells)

Full Title Evaluating the potential for lysosomal trapping in immortalized human hepatocytes (Fa2N-4 cells) Abstract Sequestration of high concentrations of lipophilic amines (a.k.a cationic amphiphilic...
Publications

Comprehensive Quantitative and Qualitative Liquid Chromatography–Radioisotope–Mass Spectrometry Analysis for Safety Testing of Tolbutamide Metabolites Without Standard Samples

Zenzaburo Tozuka, Shinsuke Aoyama, Kohei Nozawa, Shoji Akita, Toshinari Oh-Hara, Yasuhisa Adachi, Shin-Ichi Ninomiya ADME & Tox. Research Institute, SEKISUI Medical Company, Ltd., Tokai, Ibaraki...
Scientific Posters

The effect of multiple cryopreservation cycles on drug-metabolizing enzymes in human hepatocytes

Cryopreserved hepatocytes provide a convenient in vitro test system to study the phase-1 and phase-2 metabolism of new chemical entities; however cryopreservation (freeze-thaw cycles) can...
Scientific Posters

Accurate mass spectrometry elucidates a misleading metabonate formed from amine-containing drugs in reactive metabolite screening assays

Full Title Accurate mass spectrometry elucidates a misleading metabonate formed from amine-containing drugs in reactive metabolite screening assays Abstract The propensity of drug candidates to...
Scientific Posters

In situ evaluation of CYP1A2, CYP2B6 and CYP3A4/5 induction in cultured human hepatocytes with a cocktail of probe substrates: A comparison of percent positive control values

Full Title In situ evaluation of CYP1A2, CYP2B6 and CYP3A4/5 induction in cultured human hepatocytes with a cocktail of probe substrates: A comparison of percent...
Scientific Posters

The effect of multiple cryopreservation cycles on drug-metabolizing enzymes in human hepatocytes

Full Title The effect of multiple cryopreservation cycles on drug-metabolizing enzymes in human hepatocytes Abstract Cryopreserved hepatocytes provide a convenient in vitro test system to...
Scientific Posters

Pitfalls in the Design of Metabolism-Dependent CYP Inhibition (MDI) Experiments With a Dilution Step: Inhibitor Depletion by Metabolism and/or Microsomal Binding Leads to Underestimation of the Shifted IC50 Value

Full Title Pitfalls in the Design of Metabolism-Dependent CYP Inhibition (MDI) Experiments With a Dilution Step: Inhibitor Depletion by Metabolism and/or Microsomal Binding Leads to...
Scientific Posters

Effects of Gender, Age and Ethnicity on Human Cytochrome P450 Activity

Full Title Effects of Gender, Age and Ethnicity on Human Cytochrome P450 Activity Abstract It has been well documented that the expression of CYP enzymes...
Scientific Posters

Microsomes and S9 Prepared from Renal Tissue Yield High CYP, FMO and UGT Activities that are Stable…

Presented at the JSSX/ISSX Meeting, Maui, HI October 2005. Abstracts published in Drug Metabolism Reviews, Vol. 37, Supplement 2 Full Title Microsomes and S9 prepared...
Publications

Liver CYP Enzyme Activities in Selected Species

Find cytochrome P450 (CYP) enzyme activities for the following species: – Mouse – Sprague Dawley Rat – New Zealand Rabbit – Beagle Dog – Cynomolgus...
Scientific Posters

Cytochrome P450 3A4 and 1A2 Induction in Immortalized and Primary Cultures of Human Hepatocytes

Full Title Cytochrome P450 3A4 and 1A2 Induction in the Immortalized Hepatocyte Line, Fa2N-4, and Comparison with Primary Cultures of Human Hepatocytes Abstract Primary cultures...
Scientific Posters

Microsomes Prepared from Eluted Enterocytes Yield High Cytochrome P450 and UGT Activities that are…

Full Title Microsomes Prepared from Eluted Enterocytes Yield High Cytochrome P450 and UGT Activities that are Stable over Multiple Freeze/Thaw Cycles Abstract Enterocytes in the...
Scientific Posters

The Use of Immortalized Hepatocytes in Induction Studies

Abstract Primary cultures of human hepatocytes are widely used to evaluate the cytochrome P450 (CYP) enzyme-inducing potential and/or toxicity of drug candidates. However, the availability...