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Human Liver Lysosomes

Lysosomes are organelles isolated fromHuman liver tissue and are a main site of degradation, or catabolism, within the cell. Purified fractions show superior lysosomal enzymatic activity compared to microsomes and S9 with low contaminating activity from mitochondrial enzymes and can be used as an effective test system in drug development to evaluate lysosomal stability of large molecule biopharmaceuticals and macromolecules.

Our Approach to Providing Lysosomes for Catabolism Experiments

Human liver lysosomes provide a high-quality, specific, relevant, easy and ready to use in vitro test system to monitor stability/catabolism of targeted biotherapeutics. Human hepatic lysosomes are more predictive in vitro reagents than individual purified proteins, as multiple enzymes or physical characteristics may synergistically affect the stability of biomolecules.

Purified recombinant enzymes, cell homogenates or cell lines engineered to overexpress certain proteins cannot replicate the environment that compounds will encounter in the native lysosome, nor do they take into account inherent differences between primary human tissue and cell lines. Our specially prepared, purified hepatic lysosomes offer a complete and stoichiometrically correct enrichment of lysosomal contents from human primary tissue for greater lysosomal specific enzymatic activity and enrichment of lysosomal proteins with low contaminating activity from mitochondrial enzymes.

Lysosomes isolated fromHuman liver tissue can be useful in drug metabolism & catabolism assays

To ensure maximal results, our team has developed a lysosomal isolation protocol which has been optimized through characterization by immunoblot, enzymatic activity, and protease content. We have also formulated a specialized buffer recommended for use with any in vitro assay using lysosomes or tritosomes.

Animal Liver Lysosomes

We also offer tritosomes isolated from rodent hepatic cells. Rat liver tritosomes are hepatic lysosomes that have been loaded with Tyloxapol (Triton WR 1339), a non-ionic surfactant. Tyloxapol-containing lysosomes exhibit decreased density and can be more efficiently isolated away from mitochondria and contaminating cellular organelles that have overlapping densities with native lysosomes. Mouse liver tritosomes may also be prepared by our Custom Products team.

Optimize lysosomal activity with our 10x Catabolism Buffer

10x catabolism buffer has been formulated and optimized to extract the most in vitro catabolic performance from isolated human lysosomes and rat tritosomes. All of the catabolic data presented in our published scientific posters rely on this specially formulated buffer, optimized to facilitate maximum catabolic activity in lysosomal assays. Read more in our IgG Catabolism Protocol – Lysosome / Tritosome Technical Tips.

Scientific Content: In Vitro Applications of Human Liver Lysosomes

Poster: In vitro characterization of human liver lysosomes isolated from fresh tissue

Webinar: Tritosomes and Lysosomes for Characterization of Biologic Drugs

Download our IgG Catabolism Guide

Find what you need to know about IgG catabolism analysis, enzyme incubation, SDS PAGE, immunoblotting, ECL, digital imaging, and more

lysosomotropic drugs may be sequestered in lysosomes

Learn more

Access more details about how lysosomal data can help you in your drug’s development, as well as notes on methodology and results for catabolism experiments

CTA - Products 7 - Subcellular fraction test systems preparation