Tritosomes FAQ

What are tritosomes?

Tritosomes are lysosomes that have been treated with Triton WR 1339 (now called Tyloxapol). Triton WR 1339 modifies the density of the Lysosomes. Triton Lysosomes.
 

Why tritosomes?

Tritosomes were developed because lysosomes and mitochondria have overlapping densities in sucrose. Sucrose was the most common density gradient when tritosomes were first developed. Other separation materials exist now, however, they have their own set of pros and cons.

Tyloxapol is taken up by lysosomes and causes changes in lysosomal lipid composition.  This causes the tritosomes to have a lighter density than untreated lysosomes and allows improved separation from mitochondria using sucrose density gradients, resulting in a purer isolation.

Tritosomes have been continuously used for all areas of lysosome research since they were developed in the late 1960s. Tritosomes are a widely accepted technique for lysosomal isolation.

Many next generation drugs are being combined with biologics, specifically targeting sub-populations of cells (Abs and receptor ligands) or are using reversible modifications to stabilize/inactivate free serum concentration, but become activated by a lysosomal component after internalization and localization to the lysosome.
 

What are the benefits of tritosome/lysosome preparations?

Tritosome preparations are expected to have similar catabolic activity as native lysosomes.

Tritosome preparation offers a simplified system to study/predict how reagents/compounds are modified in the lysosomal compartment compared to cell culture or animal studies.

Tritosomes allow results to be obtained more quickly due to less pre-experiment/reagent preparation work.

Tritosomes are more representative than using individual purified proteins as multiple enzymes/physical characteristics may work in synergy to affect total stability/activation. For example: various ADCs are likely to have a heterogeneous drug antibody ratio (DAR) and targeted modifications that stabilize the compound while in circulation. Differing amounts of drug, conjugated to various regions of the antibody, with assorted types of linkers will undoubtedly affect stability. It is beneficial to do initial screens with the most simplified and quick assay possible, yet has the most in vivo relevance.
 

Does XenoTech offer tritosome/lysosome production for other species?

Mouse tritosomes are also available as a custom product, and Sekisui XenoTech offers the production of lysosomes from a variety of species as custom products. For more information, contact a Sekisui XenoTech Product Specialist today.

For protocol and tech tips, view our IgG Catabolism Protocol - Lysosome / Tritosome Technical Tips guide.

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