Skip to content
Molecules
SEKISUI XenoTech DD Blog Logo

NADPH RapidStart Regeneration System for Extended Metabolism

Originally published in tebu-bio’s Being Bioreactive. To purchase SEKISUI XenoTech’s products in the EU, please visit tebu-bio’s website.

NADPH is a critical cofactor supporting numerous biochemical reactions. In ADME-Tox studies, NAD(P)H regeneration is strongly recommended when using drug metabolizing enzymes (ex. Cytochrome P450 (CYP), Flavin-containing MonoOxygenases (FMO)), Recombinant CYPs (incl. bactosomes) or cellular fractions (Microsomes, S9).  Currently, the most simple and cost-effective way to regenerate the NAD(P)H in situ and enzymatically is to use the commercially-available RapidStart™ NADPH Regenerating System.

RapidStart uses an enzymatic reaction that changes NADP to NADPH, which is then oxidized by CYPs back to NADP, and the cycle continues…

RapidStart™ is a versatile system that supports extended metabolism of test articles by drug metabolizing enzymes and sub-cellular fractions for in vitro drug assays. The system is based on Glucose-6-Phosphate DeHydrogenase (G6PDH) and Glucose-6-Phosphate (G6P), with reduction of NADP+ to NADPH. A most interesting advantage is that it’s extremely simple to use – just add water and use it!

NADPH RapidStart regenerating– versatile & simple ready-to-use system

RapidStart™ NADPH regenerating system is flexible since the end-user can conveniently modulate and adjust the NADPH concentration by simply adding water to it.

Simple protocol work flow: add – agitate – activate!

  1. Determine the desired initial concentration using our RapidStart™ Dilution Concentration Tables (see below)
  2. Add the appropriate amount of high purity water in one single 0.3mL or 1.5 mL vial
  3. Vortex until pellets are fully dissolved & the NADPH regenerating system is activated and is ready for use!
RapidStart

Once activated, RapidStart™ maintains a suitable concentration of NADPH for 12 hours at room temperature or 48 hours at 2-4°C, allowing for multi-day use of the NADPH regenerating system.

Originally published in tebu-bio’s Being Bioreactive. To purchase SEKISUI XenoTech’s products in the EU, please visit tebu-bio’s website.

Learn more about:

About the Authors

Subscribe to our Newsletter

Stay up to date with our news, events and research

In vitro pipette test article treatment
005 International Messaging Created with Sketch.

Do you have a question or a request for upcoming blog content?

We love to get your feedback

A scientist utlilizing a microplate