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HepatoSure® 100-Donor PooledHuman Hepatocytes

HepatoSure® pooled donorHuman hepatocytes is the largest pool that we offer as a standard product, containing hepatocytes from 100 individual donors. HepatoSure® provides the best value when it comes to consistency of data reproducibility and predictive results representative of a large donor population.

Our 100-Donor Pool

The advantages of selecting such a  large pool representative of donor diversity include compensation for inter-individual variability and reduction in lot-to-lot variability. We offer the most extensive characterization on the market,  including transporter activities and intrinsic clearance values. Each lot of hepatocytes going into the pool is vetted by our specialists for high enzyme activity levels and the ability to maintain excellent viability for up to 4-6 hours. Pools of other sizes and characterization options can be custom-fit to meet your needs– see our CryostaX®pooled suspension hepatocytes page for more details.

Immortalized hepatocytes in lysosomal trapping assay in drug development

Characterization of Hepatocytes

For every lot of HepatoSure® suspension human hepatocytes, intrinsic clearance data is provided for CYP1A2, 2C8, 2C9, 2D6 and 3A4/5, as well as the following enzymes:

Enzyme Marker Substrate Reaction
CYP1A2 Phenacetin O-dealkylation
CYP2A6 Coumarin 7-hydroxylation
CYP2B6 Bupropion hydroxylation
CYP2C8 Amodiaquine N-dealkylation
CYP2C9 Diclofenac 4′-hydroxylation
CYP2C19 S-Mephenytoin 4′-hydroxylation
CYP2D6 Dextromethorphan O-demethylation
CYP2E1 Chlorzoxazone 6-hydroxylation
CYP3A4/5 Testosterone 6β-hydroxylation
CYP3A4/5 Midazolam 1′ -hydroxylation
UGT 7-Hydroxycoumarin glucuronidation
SULT 7-Hydroxycoumarin sulfonation
AO p-Vanillin
AO Phthalazine
OCT1 1-Methyl-4-phenylpyridinium iodine
OATP1B1 Esterone sulfate
OATP1B3 Cholecystokinin-8
NTCP Taurocholic acid

Optimizing In Vitro Assays with Hepatosure®

Our HepatoSure® Optimization kit is ideal for maximizing yield and assay quality by supplying cells with nutrient-rich media in a formula made specifically for HepatoSure® hepatocytes. Additionally, OptiThaw and OptiIncubate media are specially formulated to optimize viability, enzyme activity,  and reproducible results when performing assays with our hepatocytes in suspension. When planning your in vitro assays, pair your test systems with the right media to maximize performance and output. Read more in our Media Selection & Hepatocyte Handling guide.

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cells in vitro assay

Prepare Your Hepatocytes for Success

Review our thawing demo or download the protocol to make the most of your SEKISUI XenoTech hepatocytes

Wireframe medicine being used on a Liver