HepatoSure® pooled donorHuman hepatocytes is the largest pool that we offer as a standard product, containing hepatocytes from 100 individual donors. HepatoSure® provides the best value when it comes to consistency of data reproducibility and predictive results representative of a large donor population.
HepatoSure® 100-Donor PooledHuman Hepatocytes
HepatoSure® 100-Donor Pooled Human Hepatocytes
Our 100-Donor Pool
The advantages of selecting such a large pool representative of donor diversity include compensation for inter-individual variability and reduction in lot-to-lot variability. We offer the most extensive characterization on the market, including transporter activities and intrinsic clearance values. Each lot of hepatocytes going into the pool is vetted by our specialists for high enzyme activity levels and the ability to maintain excellent viability for up to 4-6 hours. Pools of other sizes and characterization options can be custom-fit to meet your needs– see our CryostaX®pooled suspension hepatocytes page for more details.
Characterization of Hepatocytes
For every lot of HepatoSure® suspension human hepatocytes, intrinsic clearance data is provided for CYP1A2, 2C8, 2C9, 2D6 and 3A4/5, as well as the following enzymes:
|Enzyme||Marker Substrate Reaction|
|CYP3A4/5||Midazolam 1′ -hydroxylation|
Optimizing In Vitro Assays with Hepatosure®
Our HepatoSure® Optimization kit is ideal for maximizing yield and assay quality by supplying cells with nutrient-rich media in a formula made specifically for HepatoSure® hepatocytes. Additionally, OptiThaw and OptiIncubate media are specially formulated to optimize viability, enzyme activity, and reproducible results when performing assays with our hepatocytes in suspension. When planning your in vitro assays, pair your test systems with the right media to maximize performance and output. Read more in our Media Selection & Hepatocyte Handling guide.