Single donor plateable Human hepatocytes can be used as a test system in many drug metabolism and pharmacokinetics (DMPK)-related preclinical studies (e.g. definitive enzyme induction assays, metabolic stability, integrated metabolism, uptake/efflux transporter assays, 3D hepatic models) as well as other disciplines, such as virology, cell biology, biochemistry, and genomics. We offer assured minimum yield (AMY) of 4 or 6 million cells and many selection criteria options, affording you plenty of lots to choose from to fit your study.
Single Donor Plateable Human Hepatocytes
Cryoplateable hepatocytes are the closest alternative to using fresh hepatocytes. They are unique in their ability to attach to collagen substratum and can be cultured long enough to respond to prototypical inducers even after being cryogenically frozen. Our products team qualifies every attaching lot for the ability to maintain a confluent monolayer (>85% confluency) in culture for five days, an important criterion for IND-enabling enzyme induction, toxicology, and some low-turnover compound metabolism studies. Additionally, a datasheet detailing optimal seeding density and a photomicrograph of culture accompany each lot shipment for user convenience.
Cryopreservation allows for long-term testing from the same donor while maintaining the ability to respond to prototypical inducers, maintain drug-metabolizing enzyme activities, and express transporter activity. Since these cryopreserved hepatocytes are not as time-sensitive as fresh hepatocytes, they are most commonly used for long-term metabolic stability, induction, toxicity, and transporter studies. This allows you convenience as the end-user, by not having to schedule your studies around the sporadic availability of fresh hepatocytes.
|Enzyme||Marker Substrate Reaction|
*Uptake transporter characterization available on specified lots.
*Fold Induction for CYP1A2, 2B6 and 3A4 determined by measuring mRNA levels and enzymatic activities.