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test systems

Single Donor PlateableHuman Hepatocytes

Single donor plateableHuman hepatocytes can be used as a test system in many drug metabolism and pharmacokinetics (DMPK)-related preclinical studies (e.g. definitive enzyme induction assays, metabolic stability, integrated metabolism, uptake/efflux transporter assays, 3D hepatic models) as well as other disciplines, such as virology, cell biology, biochemistry, and genomics. We offer assured minimum yield (AMY) of 4 or 6 million cells and many selection criteria options, affording you plenty of lots to choose from to fit your study.

Cryoplateable hepatocytes are the closest alternative to using fresh hepatocytes. They are unique in their ability to attach to collagen substratum and can be cultured long enough to respond to prototypical inducers even after being cryogenically frozen. Our products team qualifies every attaching lot for the ability to maintain a confluent monolayer (>85% confluency) in culture for five days, an important criterion for IND-enabling enzyme induction, toxicology, and some low-turnover compound metabolism studies. Additionally, a datasheet detailing optimal seeding density and a photomicrograph of culture accompany each lot shipment for user convenience.

Cryopreservation allows for long-term testing from the same donor while maintaining the ability to respond to prototypical inducers, maintain drug-metabolizing enzyme activities, and express transporter activity. Since these cryopreserved hepatocytes are not as time-sensitive as fresh hepatocytes, they are most commonly used for long-term metabolic stability, induction, toxicity, and transporter studies. This allows you convenience as the end-user, by not having to schedule your studies around the sporadic availability of fresh hepatocytes.

Hepatocyte Characterization

EnzymeMarker Substrate Reaction
CYP1A2Phenacetin O-dealkylation
CYP2A6Coumarin 7-hydroxylation
CYP2B6Bupropion hydroxylation
CYP2C8Amodiaquine N-dealkylation
CYP2C9Diclofenac 4′-hydroxylation
CYP2C19S-Mephenytoin 4′-hydroxylation
CYP2D6Dextromethorphan O-demethylation
CYP2E1Chlorzoxazone 6-hydroxylation
CYP3A4/5Testosterone 6β-hydroxylation
CYP3A4/5Midazolam 1′-hydroxylation
 UGT7-Hydroxycoumarin glucuronidation
SULT7-Hydroxycoumarin sulfonation
OCT1*1-Methyl-4-phenylpyridinium iodine
NTCP*Taurocholic acid

*Uptake transporter characterization available on specified lots.
*Fold Induction for CYP1A2, 2B6 and 3A4 determined by measuring mRNA levels and enzymatic activities.

Optimize your assays with the right media

OptiThaw and OptiPlate media are specially formulated by in vitro product experts to maintain higher viability during thawing and preparation for culture and are recommended for use with our plateable lots for optimum results. Be sure to also follow our thawing and plating demos for best practice using our cryopreserved primary hepatocytes:

Make sure you get the most out of your hepatocytes

When you purchase from us, we make sure you have all the right tools and information you need to succeed

Bioanalytical Services Web Lab

Build Your Own Pool

We offer custom pooling for no additional cost! You can select attaching or suspension lots, pool size, donor criteria, enzyme activity characterization, and more

“Isolation of subcellular fractions: Tissue > Homogenate > S9 > Cytosol > Microsomes