Subcellular Fractions

Subcellular fractions are widely used in drug discovery and preclinical drug development to evaluate the in vitro metabolism of drug candidates. Metabolic stability, in vitro intrinsic clearance, reaction phenotyping and CYP/UGT enzyme inhibition can all be evaluated with subcellular fractions.
 
The primary subcellular fractions used to support these studies are hepatic and extrahepatic tissue microsomes, S9, cytosol, homogenate and mitochondria. Lysosomes are also available. Sekisui XenoTech offers these fractions from humans and toxicologically relevant species.


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Standard Subcellular Fraction Products:


If you require microsomes, S9, cytosol, homogenate, lysosomes or mitochondria not available through our standard product offerings, custom subcellular fractions can be easily ordered to meet the specific needs of any study. Contact our dedicated product specialists to find out how Sekisui XenoTech's custom products can meet your needs.

Liver, Lung and Kidney Subcellular Fraction Isolation Procedure:
Subcellular Fraction Preparation Method
Tissue > Homogenate > S9 > Cytosol > Microsomes

Subcellular Fraction Scientific Resources
Poster: UGT Inhibition Studies in the Presence or Absence of Alamethicin
Poster: Evaluation of Chemical Inhibitors for UDP-glucuronosyltransferase (UGT) Reaction Phenotyping Assays in Human Liver Microsomes
Poster: Assessment Under Initial Rate Conditions of the Selectivity and Time Course of Cytochrome P450 Inactivation in Pooled Human Liver Microsomes and Hepatocytes
Poster: The Effect of Buffer Ionic Strength or Various Media on the In Vitro Metabolism of Cytochrome P450 Substrates in Pooled Human Liver Microsomes and Cryopreserved Human Hepatocytes
Paper: In vitro inhibition of human liver cytochrome P450 (CYP) and UDP-glucuronosyltransferase (UGT) enzymes by rose bengal
Poster: Selection of human liver S9 and cytosol fractions for evaluating clearance by aldehyde oxidase (AO)
Poster: Test system-dependent clearance of CYP2D6 and CYP3A4/5 substrates
Poster: Human liver S9 fractions stored at -70°C maintain high phase I and phase II enzymatic activities over multiple freeze/thaw cycles and for at least 10 years
Poster: Midazolam clearance in human hepatocytes is restricted compared with human liver microsomes but not by cell permeability or cofactor availability
Poster: In vitro inhibition and induction of human liver cytochrome P450 enzymes by NTBC and its metabolism in human liver microsomes
Poster: Inhibitor Depletion by Metabolism and/or Microsomal Binding Leads to Underestimation of the Shifted IC50 Value

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