Author: Dr. Chris Bohl
Which Hepatocytes Should I Use for What Studies?
- Test Systems & Methods
- March 25, 2022
- Dr. Chris Bohl, Michael Millhollen, Isis Smith
Primary hepatocytes are considered the gold standard for ADME/DMPK studies because they are the most representative in vitro test system. However, not all hepatocyte formats…
Notes from the 2021 HRA Expert Panel Discussion “Are Standard Hepatocyte Models/Test Systems Still Good Enough?”
- Test Systems & Methods
- December 7, 2021
- Dr. Chris Bohl
MicroPhysiological Systems (MPS) is a general term used to describe miniaturized, in vitro test systems that couple microfluidics with advanced...
How to Choose the Right Test Systems for Your DMPK Studies
- Test Systems & Methods
- September 12, 2019
- Dr. Chris Bohl, Madison Esely-Kohlman
Test systems for DMPK in vitro studies are part of the very foundation of our company. Our labs were borne of...
Cell Handling & Media Selection for Best Results in Hepatocyte Assays
- Test Systems & Methods
- June 19, 2019
- Dr. Chris Bohl, Madison Esely-Kohlman
Getting reliable, reproducible results from studies using hepatocytes as a model system for drug metabolism is in part dependent on how well...
Choosing a Relevant Small Animal Model for Pharmacokinetic or Toxicity Studies
- Test Systems & Methods
- March 1, 2018
- Dr. Chris Bohl
Choosing the most suitable small animal model is crucial for pharmacokinetic and/or toxicology studies in order to get usable, relevant data. Due to potential...
What To Do with Microsome Stable Low Turnover Compounds
- Drug Metabolism
- December 7, 2017
- Dr. Chris Bohl
Compounds that exhibit high metabolic stability in hepatocytes and subcellular fractions (S9, microsomes and cytosol) can be a challenge for ADME scientists. These in...
Considerations When Studying Esterase Activities in the Intestine
- Drug Metabolism
- July 12, 2017
- Dr. Chris Bohl, Michael Millhollen
The gastrointestinal wall is a significant site of first-pass metabolism for oral drugs, which is commonly associated with CYP450 and...
New Lysosome Catabolism Protocol and Tech Tips
- Biologics & Oligonucleotides
- April 17, 2017
- Dr. Chris Bohl
A new guide outlining our IgG Catabolism Protocol as well as Lysosome and Tritosome Technical Tips is now available for those evaluating lysosomal stability...
XenoTech Standardizes Lysosomal Test Systems for Biopharmaceutical Development
- Test Systems & Methods
- October 5, 2016
- Dr. Chris Bohl
Before releasing these test systems, there was not a high quality, specific, relevant, easy and ready to use, in vitro test system to...
Why Switch to Hepatocyte Pellets?
- Test Systems & Methods
- June 30, 2021
- Michael Millhollen, Dr. Chris Bohl
You may have heard about the patented CryostaX® format of hepatocyte pellets, but do you know why XenoTech and countless other researchers have made the…
Timing of In Vitro Studies: Early, Thorough ADME for Your Compound’s Success
- Regulatory Guidance
- February 13, 2020
- Madison Esely-Kohlman, Dr. Chris Bohl, Greg Loewen
Beyond the need for evidence that a drug works, Food and Drug Administration (FDA) and other regulatory bodies around the...
Disease-State Test Systems
- Test Systems & Methods
- January 13, 2019
- Michael Millhollen, Dr. Chris Bohl
XenoTech has been working with and supplying reagents for the pre-clinical ADME field for well over two decades. Years of...
No-Cost, On-Demand Primary Human Hepatocyte Pooling Using CryostaX Pellets
- Test Systems & Methods
- June 15, 2018
- Michael Millhollen, Dr. Chris Bohl
CryostaX® hepatocytes are created using a patented process that produces unique single-donor cell pellets. This format allows scientists to easily pool primary...
Maximize Metabolic Activities by Limiting Hepatocyte Cryoinjury
- Drug Metabolism
- May 1, 2018
- Michael Millhollen, Dr. Chris Bohl
Cryopreservation and thawing of primary hepatocytes causes inherent damage to the cells. Traditional pooling methods require cells to undergo two separate cryopreservation...
No More Water Baths! Simplify Hepatocyte Thaws and Eliminate Contamination
- Test Systems & Methods
- April 3, 2018
- Michael Millhollen, Dr. Chris Bohl
XenoTech has patented technology that eliminates the need for the water baths that are traditionally required to thaw cryopreserved hepatocytes. By removing water...