Guide to Selecting In Vitro Test Systems for Pre-Clinical Studies

Estore-Coffee-Promo.jpg

Sekisui XenoTech’s Products Supporting Preclinical DMPK Research
In order to assist you in selecting the appropriate test systems for your study, we have listed many of our products below, rated according to common in vitro study types. The section below the study-product rating tables then lists the specific test systems that we have available for each category.
 
Study Type In Vitro Test System: Hepatic Cells
  Plateable Cryopreserved Hepatocytes Pooled Cryopreserved Hepatocytes Single Donor Cryopreserved Hepatocytes Kupffer Cells
Species Differences + +++ ++
Metabolic Stability +++ +++ ++
In Vitro Toxicity ++ +
Enzyme Induction +++
P450 Inhibition + ++ +
Reaction Phenotyping + + +
Integrated Metabolism +++ +++ ++
Uptake Transporter Assays +++ +++ ++
Efflux Transporter Assays +++
Genetic Polymorphisms + +++ +
3D Hepatic Models ++ ++ ++
+++ = Preferred/Best    ++ = Good    + = Acceptable    – = Not Recommended

 
Hepatic Cells | Subcellular Fractions | Recombinant Enzymes
Study Type In Vitro Test System: Subcellular Fractions
  Pooled S9/Cytosol/ Homogenate Pooled Microsomes Single Donor Microsomes/ S9/Cytosol/ Lysosomes/ Tritosomes
Species Differences ++ ++ + ++
Metabolic Stability ++ ++ + +++
In Vitro Toxicity +++ +
Enzyme Induction
P450 Inhibition + +++ +
Reaction Phenotyping ++ +++ +
Integrated Metabolism ++ ++ + +
Uptake Transporter Assays
Efflux Transporter Assays
Genetic Polymorphisms ++
3D Hepatic Models
+++ = Preferred/Best    ++ = Good    + = Acceptable    – = Not Recommended

 
Hepatic Cells | Subcellular Fractions | Recombinant Enzymes
Study Type In Vitro Test System: Recombinant CYPs
  Recombinant Enzymes (rCYPS)
Species Differences
Metabolic Stability +
In Vitro Toxicity
Enzyme Induction
P450 Inhibition +
Reaction Phenotyping ++
Integrated Metabolism
Uptake Transporter Assays
Efflux Transporter Assays
Genetic Polymorphisms +
3D Hepatic Models
+++ = Preferred/Best   ++ = Good   + = Acceptable   – = Not Recommended
  Find the Cellular Products You Need  
Can't find what you need? Have a question?
Or Call 1-877-588-7530
(+1.913.438.7450 If Outside North America)

   
In Vitro Study Type Available Sekisui XenoTech Products
Enzyme Induction Cryoplateable human/animal hepatocytes,
CryostaX™ plateable pooled hepatocytes,
Fresh human/animal hepatocytes,
Ready- Plate™ human hepatocytes,
Treated animal liver microsomes
Hepatic Uptake HepatoSure™ pooled human hepatocytes (n=100),
CryostaX™ pooled human hepatocytes (n=10/20),
CryostaX™ plateable pooled hepatocytes (n=5/10),
Cryoplateable human hepatocytes,
Transporter certified human hepatocytes
Hepatic Efflux Transporter certified human hepatocytes,
CryostaX™ plateable pooled hepatocytes,
Cryoplateable human hepatocytes
Enzyme Inhibition Human liver microsomes: XTreme 200-donor pool, 
Human liver S9, cytosol: XTreme 200-donor pool,
HepatoSure™ pooled human hepatocytes,
CryostaX™ human hepatocytes,
Cryoplateable human/animal hepatocytes,
Ready-Plate™ human hepatocytes
Recombinant enzymes/bactosomes (rCYPs)
CYP inhibitors/substrates
Metabolic Stability/Clearance Human liver microsomes: XTreme 200 or 50-donor pool,
Human liver S9: XTreme 200 or 50-donor pool,
Human liver homogenate,
HepatoSure™ pooled human hepatocytes,
CryostaX™ human hepatocytes,
Cryoplateable human/animal hepatocytes,
Ready- Plate™ human hepatocytes,
Animal microsomes, S9, extrahepatic subcellular fractions,
Lysosomes/tritosomes
Metabolite Characterization Human liver microsomes: XTreme 200-donor pool, 
Human liver S9: XTreme 200-donor pool,
Human liver homogenate
HepatoSure™ pooled human hepatocytes,
CryostaX™ pooled human hepatocytes,
Animal cryopreserved/fresh hepatocytes
Species Comparison Human, monkey, dog, minipig, rabbit, guinea pig, hamster, rat, mouse, etc. microsomes, S9 and hepatocytes
Reaction Phenotyping Human liver microsomes: XTreme 200 or 50-donor pool,
Reaction phenotyping kit,
Pooled human hepatocytes
Recombinant enzymes/bactosomes (rCYPs)
Reactive Metabolites Human liver microsomes: XTreme 200 or 50-donor pool,
Human liver S9: XTreme 200 or 50-donor pool,
Human liver homogenate,
HepatoSure™ pooled human hepatocytes,
CryostaX™ human hepatocyte pools,
Human/animal cryopreserved/fresh hepatocytes
Lysosomal Trapping HepatoSure™ pooled human hepatocytes,
Cryoplateable human hepatocytes,
CryostaX™ plateable pools of human hepatocytes,
Fa2N-4 immortalized hepatocytes
Kupffer Cell Mediated Toxicity/Macrophage Stimulation Kupffer cells/Kupffer cell thawing and culture media
Polymorphic Enzymes Geneknown™ human hepatocytes – CYP2C8, CYP2C9, CYP2C19, CYP2D6, C YP3A5, UGT1A1, OATP1B1 Genotyped human liver microsomes – CYP2C9, CYP2C19, CYP2D6, CYP3A5, UGT1A1, UGT1A9
Gender Differences / Demographic Differences Male/female specific lots of liver microsomes, var. species
Individual donor human liver microsomes and hepatocytes Ethnic and age specific pools available
Enzyme Cofactors RapidStart™ NADPH regenerating system
Hepatocyte Media Cryopreservation thawing kits, resuspension medium, culture medium and incubation medium
Fresh Hepatocytes Fresh plated human/animal hepatocytes 6, 12, 24, 48 and 96-plate well formats
Fresh human/animal suspensions
Custom Products Human/animal cellular and subcellular products, various tissues/organs. By request only.

  Find the Cellular Products You Need  
Can't find what you need? Have a question?
Or Call 1-877-588-7530
(+1.913.438.7450 If Outside North America)

 
Contact Us

Contact Us

Questions or inquiries? Use our contact form and we’ll reply as soon as possible.* indicates required field

Request A Quote
Request A Quote

Request A Quote

 Security code